Allylamine-2’-deoxyuridine-5’-triphosphate can replace TTP in reactions where it serves as a substrate for E. coli DNA polymerase 1 (holoenzyme and Klenow fragment), terminal deoxynucloetide transferase, T4 and Taq DNA polymerases, and reverse transcriptases (from AMV and M-MuLV). Allylamine-dUTP is incorporated into DNA by a variety of labeling reactions including nick translation, random primed DNA synthesis and 3’ end labeling reactions. Allylamine-labeled DNA can be efficiently conjugated to the active ester form of signal generating moieties such as fluorescent dyes, biotin and other haptens to produce labeled probes for hybridization/detection assays. The efficient incorporation of Allylamine-dUTP, in contrast to many dye-labeled nucleotides, provides for greater incorporation of signaling moieties and stronger signals.
Product Details
Alternative Name: | Allylamino-2’-deoxyuridine-5’-triphosphate |
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Formula: | C12H20N3O14P3 |
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MW: | 523.2 (free acid) |
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Concentration: | 50mM |
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Formulation: | Liquid. Solution in water. |
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Extinction Coefficient: | 8,100 M-1 cm-1 (290 nm, water) |
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Purity: | ≥93% (HPLC) |
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Purity Detail: | Purified by ion-exchange chromatography. |
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Appearance: | Clear, colorless solution, free of solid particulates. |
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Shipping: | Dry Ice |
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Long Term Storage: | -20°C |
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Handling: | Avoid freeze/thaw cycles. |
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Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Lineage-specific RUNX2 super-enhancer activates MYC and promotes the development of blastic plasmacytoid dendritic cell neoplasm: S. Kubota, et al.; Nat. Commun.
10, 1653 (2019),
Abstract;
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