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Allylamine-dUTP

 
ENZ-42861 2.5 µmol 155.00 USD
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Allylamine-2’-deoxyuridine-5’-triphosphate can replace TTP in reactions where it serves as a substrate for E. coli DNA polymerase 1 (holoenzyme and Klenow fragment), terminal deoxynucloetide transferase, T4 and Taq DNA polymerases, and reverse transcriptases (from ALV and MLV). Allylamine-dUTP is incorporated into DNA by a variety of labeling reactions including nick translation, random primed DNA synthesis and 3’ end labeling reactions. Allylamine-labeled DNA can be efficiently conjugated to the active ester form of signal generating moieties such as fluorescent dyes, biotin and other haptens to produce labeled probes for hybridization/detection assays. The efficient incorporation of Allylamine-dUTP, in contrast to many dye-labeled nucleotides, provides for greater incorporation of signaling moieties and stronger signals.

Product Specification

Alternative Name:Allylamino-2’-deoxyuridine-5’-triphosphate
 
Formula:C12H20N3O14P3
 
MW:523.2 (free acid)
 
Concentration:50mM
 
Formulation:Liquid. Solution in water.
 
Extinction Coefficient:8,100 M-1 cm-1 (290 nm, water)
 
Purity:≥93% (HPLC)
 
Purity Detail:Purified by ion-exchange chromatography.
 
Appearance:Clear, colorless solution, free of solid particulates.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
 
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