Red 598 dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labelled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 598 dUTP can also be used for multicolor fluorescence labelling.
Product Details
| Concentration: | 1mM |
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| Formulation: | Liquid. Solution in water. |
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| Excitation maximum: | 598nm |
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| Emission maximum: | 610nm |
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| Extinction Coefficient: | 119,000 M-1 cm-1 (598 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA]) |
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| Purity: | ≥93% (HPLC) |
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| Quality Control: | Red 598 dUTP is purified by Ion-Exchange Chromatography. HPLC is used as a quality control to ensure chemical purity of ≥93%. The UV/VIS absorption maximum is obtained in aqueous Tris buffer to determine concentration. |
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| Appearance: | Purple liquid. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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| Regulatory Status: | RUO - Research Use Only |
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