Red 580 [5-ROX] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 580 dUTP can also be used for multicolor fluorescence labeling.
This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).
Product Details
Alternative Name:
5-ROX dUTP
Quantity:
Sufficient for approximately 49 reactions, following the recommended protocol of Prod. No. ENZ-GEN111.
Concentration:
1mM
Formulation:
Liquid. Solution in water.
Excitation maximum:
580 nm
Emission maximum:
603 nm
Extinction Coefficient:
75,000 M-1 cm-1 (580 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])
Correction Factor (260 nm):
0.44
Correction Factor (280 nm):
0.40
Purity:
≥93% (HPLC)
Appearance:
Purple liquid.
Applications:
FISH
Shipping:
Dry Ice
Long Term Storage:
-20°C
Handling:
Protect from light. Avoid freeze/thaw cycles.
Technical Info/Product Notes:
Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
Regulatory Status:
RUO - Research Use Only
Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.
Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.
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Product Literature References
Highly Multiplexed Fluorescence in Situ Hybridization for in Situ Genomics: M.L. Onozato, et al.; J. Mol. Diagn. 21, 390 (2019), Application(s): Multiplex 2 and 3 colors in combination with Aqua 431-dUTP, Green 496-dUTP, Orange 552-dUTP, Gold 525-dUTP, Red 650 (Cy5)-dUTP, Abstract; Full Text
In-cell identification and measurement of RNA-protein interactions: A. Graindorge, et al.; Nat. Commun. 10, 5317 (2019), Abstract; Full Text
Structural and spatial chromatin features at developmental gene loci in human pluripotent stem cells: H. Ikeda, et al.; Nat. Commun. 8, 1616 (2017), Application(s): Generation of FISH probes, Abstract; Full Text
Subtyping of renal cortical neoplasms in fine needle aspiration biopsies using a decision tree based on genomic alterations detected by fluorescence in situ hybridization: B. Gowrishankar, et al.; BJU Int. 114, 881 (2014), Abstract; Full Text
