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Orange 552 dUTP (lyophilized)

 
ENZ-42842L-0050 50 nmol 475.00 USD
Do you need bulk/larger quantities?
 
For ready-to-use, aqueous format, see ENZ-42842.
Orange 552 dUTP (5-TAMRA-dUTP) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Orange 552 dUTP can also be used for multicolor fluorescence labeling.

This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111) and is sufficient for approximately 98 reactions, following the recommended protocol.

Product Specification

Alternative Name:5-TAMRA dUTP
 
Quantity:Sufficient for approximately 98 reactions, following the recommended protocol of Prod. No. ENZ-GEN111.
 
Formulation:Lyophilized.
 
Absorbance maximum:552 nm
 
Emission maximum:576 nm
 
Extinction Coefficient:60,000 M-1 cm-1 (552 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])
 
Correction Factor (260 nm):0.27
 
Correction Factor (280 nm):0.30
 
Purity:≥93% (HPLC)
 
Purity Detail:Purified by ion-exchange chromatography.
 
Appearance:Purple solid.
 
Applications:FISH
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
 
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Product Literature References

Anolis sex chromosomes are derived from a single ancestral pair: T. Gamble, et al.; Evolution 68, 1027 (2014), Abstract;

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