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Fluorescein-12-ddUTP

 
ENZ-42833 25 nmol 311.00 USD
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Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labeled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.

Product Specification

Alternative Name:Fluorescein-12-2’,3’-dideoxyuridine-5’-triphosphate
 
Formula:C39H41N4O20P3
 
MW:978.7 (free acid)
 
Concentration:1mM
 
Formulation:Liquid. Solution in water.
 
Absorbance maximum:496 nm
 
Emission maximum:520 nm
 
Extinction Coefficient:85,000 M-1 cm-1 (496 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])
 
Purity:≥93% (HPLC)
 
Purity Detail:Purified by ion-exchange chromatography.
 
Appearance:Yellow-green liquid and orange frozen solution.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
 
ENZ-42833 structure
enz-42833
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Product Literature References

Preparation and biomedical applications of programmable and multifunctional DNA nanoflowers: Y. Lv, et al.; Nat. Protoc. 10, 1508 (2015), Application(s): Labeling of cDNA, Abstract;

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