Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labeled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.
Product Details
| Alternative Name: | Fluorescein-12-2’,3’-dideoxyuridine-5’-triphosphate |
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| Formula: | C39H41N4O20P3 |
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| MW: | 978.7 (free acid) |
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| Concentration: | 1mM |
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| Formulation: | Liquid. Solution in water. |
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| Excitation maximum: | 496 nm |
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| Emission maximum: | 520 nm |
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| Extinction Coefficient: | 85,000 M-1 cm-1 (496 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA]) |
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| Purity: | ≥93% (HPLC) |
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| Purity Detail: | Purified by ion-exchange chromatography. |
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| Appearance: | Yellow-green liquid and orange frozen solution. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Preparation and biomedical applications of programmable and multifunctional DNA nanoflowers: Y. Lv, et al.; Nat. Protoc.
10, 1508 (2015),
Application(s): Labeling of cDNA,
Abstract;
