Bio-7-dATP



ENZ-42819	50 nmol	409.00 USD

Bio-7-dATP (Biotin-7-2’-deoxyadenosine-5’-triphosphate) can replace dATP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Bio-7-dATP can be used to produce biotinylated DNA probes in a variety of labelling reactions including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. The resulting biotin-labeled probe can be used in a variety of hybridization applications incuding Southern blots, Northern blots, or dot blots.

Alternative Name:	Biotin-7-2’-deoxyadenosine-5’-triphosphate

Concentration:	1mM

Purity Detail:	Contains <5% Bio-7-dADP.

Formula:	C₂₆H₄₃N₇O₁₄P₃S

MW:	816.7 (free acid)

Purity:	≥93% (HPLC)

Appearance:	Clear, colorless liquid.

Formulation:	Liquid. Solution in water.

Long Term Storage:	-20°C

Handling:	Avoid freeze/thaw cycles.

Background / Technical Information:	Extinction Coefficient: 17,000M^-1cm^-1 (265nm, pH 7.0) Enzo is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.