Bio-11-CTP (Biotin-11-cytidine-5’-triphosphate) can replace CTP for in vitro transcription reaction catalyzed by T3, T7 or SP6 RNA polymerases. The biotin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis. The transcription reaction produces multiple RNA copies of the DNA template(s) during a short incubation period. RNA probes offer higher target specificity and greater sensitivity than the corresponding DNA-DNA hybrids. The single-stranded RNA probes offer slectivity unavailable with double-stranded DNA probes, because they are strand-specific. Furthermore, RNA probes hybridize much more efficiently to target molecules than DNA probes because there is no self-hybridization. The biotin-labeled hybridized probes can be detected by a reporter molecule linked to streptavidin, avidin, or anit-biotin antibody. Such a complex can be detected directly, e.g. by excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble colored precipitate.
Product Details
| Alternative Name: | Biotin-11-cytidine-5’-triphosphate |
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| Formula: | C28H46N7O17P3S |
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| MW: | 877.7 (free acid) |
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| Concentration: | 10mM |
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| Formulation: | Liquid. Solution in water. |
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| Extinction Coefficient: | 9,100 M-1 cm-1 (271 nm, pH 7.0) |
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| Purity: | ≥93% (HPLC) |
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| Purity Detail: | Contains <5% Bio-11-CDP. |
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| Appearance: | Clear, colorless liquid. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Handling: | Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Loss of RNA binding protein HuD facilitates the production of the senescence-associated secretory phenotype: S. Ryu, et al.; Cell Death Dis.
13, 329 (2022),
Abstract;
RNA binding protein HuD mediates the crosstalk between β cells and islet endothelial cells by the regulation of Endostatin and Serpin E1 expression: M. Jung, et al.; Cell Death Dis.
13, 1019 (2022),
Abstract;
Metagenomic shotgun sequencing reveals host species as an important driver of virome composition in mosquitoes: P. Thongsripong, et al.; Sci. Rep.
11, 8448 (2021),
Abstract;
SRSF3 Is a Critical Requirement for Inclusion of Exon 3 of BIS Pre-mRNA: J.Y. Baek, et al.; Cells
9, 2325 (2020),
Application(s): Biotin Pull-Down Assay,
Abstract;
Full Text
Polypyrimidine tract-binding protein 1-mediated down-regulation of ATG10 facilitates metastasis of colorectal cancer cells: Y. K. Jo, et al.; Cancer Lett.
385, 21 (2017),
Abstract;
Heterogeneous nuclear ribonucleoprotein A1 post-transcriptionally regulates Drp1 expression in neuroblastoma cells: S.J. Park, et al.; Biochim. Biophys. Acta
1849, 1423 (2015),
Application(s): Biotin pull-down assay,
Abstract;
Preparation of mRNA for expression monitoring: M.C. Byrne, et al.; Curr. Protoc. Neurosci.
Chapter 4, Unit 4.26 (2001),
Abstract;
