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Bio-17-ATP

 
ENZ-42817 250 nmol 250.00 USD
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Bio-17-ATP (Biotin-17-adenosine-5’-triphosphate) can replace ATP for in vitro transcription reaction catalyzed by T3, T7 or SP6 RNA polymerases. The biotin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis. The transcription reaction produces multiple RNA copies of the DNA template(s) during a short incubation period. RNA probes offer higher target specificity and greater sensitivity than the corresponding DNA-DNA hybrids. The single-stranded RNA probes offer slectivity unavailable with double-stranded DNA probes, because they are strand-specific. Furthermore, RNA probes hybridize much more efficiently to target molecules than DNA probes because there is no self-hybridization. The biotin-labeled hybridized probes can be detected by a reporter molecule linked to streptavidin, avidin, or anit-biotin antibody. Such a complex can be detected directly, e.g. by excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble colored precipitate.

Product Specification

Alternative Name:Biotin-17-adenosine-5’-triphosphate
 
Formula:C34H57N10O17P3S
 
MW:1002.8 (free acid)
 
Concentration:10mM
 
Formulation:Liquid. Solution in water.
 
Extinction Coefficient:18,000 M-1 cm-1 (267 nm, pH 7.0)
 
Purity:≥93% (HPLC)
 
Purity Detail:Contains <5% Bio-17-ADP.
 
Appearance:Clear, colorless liquid.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.

 
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