Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labeling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labeled oligonucleotides produced by this reaction are suitable as hybridization probes. Biotin-labeled cDNA produced by this reaction can be used in nucleic acid array assays. Biotin-labeled DNA in hybridization assays can be detected by a reporter molecule linked to streptavidin, avidin, or and anti-biotin antibody. Such a complex can be detected directly, e.g. by the excitation of a fluorophore conjugated to streptavidin, or indirectly, e.g. using an enzyme conjugate that can produce an insoluble colored precipitate. Bio-16-ddUTP is also a substrate for Taq DNA Polymerase and other DNA polymerases.
Product Details
| Alternative Name: | Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate . 4Li |
| |
| Formula: | C32H48Li4N7O17P3S |
| |
| MW: | 927.7 . 28.0 |
| |
| Concentration: | 1mM |
| |
| Formulation: | Liquid. Solution in water. |
| |
| Extinction Coefficient: | 10,700 M-1 cm-1 (240 nm, pH 7.0) |
| |
| Purity: | ≥93% (HPLC) |
| |
| Purity Detail: | Contains <5% Bio-16-ddUDP. |
| |
| Appearance: | Clear, colorless liquid. |
| |
| Shipping: | Dry Ice |
| |
| Long Term Storage: | -20°C |
| |
| Handling: | Avoid freeze/thaw cycles. |
| |
| Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Please mouse over
