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Bio-16-dUTP

 
ENZ-42811 50 nmol 375.00 USD
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Replaces Prod. #: ENZ-42806

Bio-16-dUTP (Biotin-16-2’-deoxyuridine-5’-triphosphate) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Bio-16-dUTP can be used to produce biotinylated DNA probes in a variety of labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. The resulting biotin-labeled probe can be used in a variety of hybridization applications incuding Southern blots, Northern blots, or dot blots. The probes can also be used for in situ hybridization procedures on fixed cells and tissues. The biotinylated probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probes can be detected by their interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color-producing enzymes.

This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).

Product Specification

Alternative Name:Biotin-16-2’-deoxyuridine-5’-triphosphate
 
Formula:C32H52N7O18P3S
 
MW:947.7 (free acid)
 
Quantity:Sufficient for approximately 50 reactions, following the recommended protocol of Prod. No. ENZ-GEN111.
 
Concentration:1mM
 
Formulation:Liquid. Solution in water.
 
Extinction Coefficient:10,700 M-1 cm-1 (240 nm, pH 7.0)
 
Purity:≥93% (HPLC)
 
Purity Detail:Contains <5% Bio-16-dUDP.
 
Appearance:Clear, colorless liquid.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:The Bio-16 dUTP linker that physically connects Biotin to the nucleotide is longer than that of Bio-11 dUTP. The accessibility of the biotin moiety is influenced by the linker length allowing more favorable interaction with biotin-binding proteins.

Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
 
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Product Literature References

Pivotal role of inosine triphosphate pyrophosphatase in maintaining genome stability and the prevention of apoptosis in human cells: M.R. Menezes, et al.; PLoS One 7, e32313 (2012), Abstract; Full Text
Bcl-2 overexpression in PhIP-induced colon tumors: cloning of the rat Bcl-2 promoter and characterization of a pathway involving beta-catenin, c-Myc and E2F1: Q. Li, et al.; Oncogene 26, 6194 (2007), Abstract; Full Text
ERK8, a new member of the mitogen-activated protein kinase family: M.K. Abe, et al.; J. Biol. Chem. 277, 16733 (2002), Abstract; Full Text

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