Bio-16-dUTP



ENZ-42811	50 nmol	409.00 USD

Replaces Prod. #: ENZ-42806

Bio-16-dUTP (Biotin-16-2’-deoxyuridine-5’-triphosphate) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Bio-16-dUTP can be used to produce biotinylated DNA probes in a variety of labelling reactions including nick translation, random prime labelling, cDNA labelling and 3’-end labelling. The resulting biotin-labeled probe can be used in a variety of hybridization applications incuding Southern blots, Northern blots, or dot blots.

Alternative Name:	Biotin-16-2’-deoxyuridine-5’-triphosphate

Concentration:	1mM

Purity Detail:	Contains <5% Bio-16-dUDP.

Formula:	C₃₂H₅₂N₇O₁₈P₃S

MW:	947.7 (free acid)

Purity:	≥93% (HPLC)

Appearance:	Clear, colorless liquid.

Formulation:	Liquid. Solution in water.

Long Term Storage:	-20°C

Handling:	Avoid freeze/thaw cycles.

Background / Technical Information:	The Bio-16 dUTP linker that physically connects Biotin to the nucleotide is longer than that of Bio-11 dUTP. The accessibility of the biotin moiety is influenced by the linker length allowing more favorable interaction with biotin-binding proteins. Extinction Coefficient: 10,700M^-1cm^-1 (240nm, pH 7.0) Enzo is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.