Bio-16-ddUTP dideoxynucleotide pack for 3’-oligonucleotide labeling

An oligonucleotide can be synthesized using standard, commercially available reagents and labeled after synthesis in a simple and reproducible enzyme reaction.The BioProbe^® 3’-Oligonucleotide Labeling System provides maximum flexibility for labeling oligonucleotides approximately 15 to 100 nucleotides in length. A standard reaction will label up to 100 picomoles (1µg of a 30-mer oligonucleotide sequence). A complete system combines a standard Reagent Pack (buffers and enzyme) (Prod. No. ENZ-42730) with a Nucleotide Pack. All components contain sufficient reagents for 25 labeling reactions.

The system is available in two modular formats:

3’-Oligonucleotide Tailing (with a "tail" of nucleotides) 3’-Oligonucleotide Labeling (with a single nucleotide)

The BioProbe^® 3’-Oligonucleotide Labeling System Dideoxynucleotide Pack contains a vial of modified dideoxynucleotide and a vial of control oligonucleotide labeled with the modified dideoxynucleotide. Terminal labeling reactions using dideoxynucleotides result in the addition of a single nucleotide at the 3’-OH terminus. For optimum results, the nucleotide contained in this Pack should be used with the 3’-Oligonucleotide Labeling System Reagent Pack.