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 United States

BioProbe® 3’-Oligonucleotide labeling system reagent pack

 
ENZ-42730 1 Pack 25 reactions 313.00 USD
 
The procedure for labeling of DNA probes with a polynucleotide "tail" containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions. Terminal labeling is an ideal procedure for the labeling of oligonucleotides.

Product Specification

Kit/Set Contains:

5X Reaction Buffer, 100 μl Potassium cacodylate buffer, pH 7, containing β-mercaptoethanol 10X CoCl2 Solution, 50 μl 10mM solution Terminal Deoxynucleotide Transferase, 50 μl 30 units/μl in storage buffer Control Oligonucleotide (unlabeled), 25 μl 0.2 μg/μl in TE Buffer (30-mer, 5’-TTG GGT AAC GCC AGG GTT TTC CCA GTC ACG-3’, homologous to the lac Z’ region of pUC-type vector DNAs)Control Target DNA, 25 μl 0.2 μg/μl in TE Buffer (Restriction enzyme digest of a pUC-type vector containing an Epstein-Barr Virus DNA insert)

 
Long Term Storage:-20°C
 
Background / Technical Information:

Enzo is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.For the Manual please click here.

 

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