Random primed labeling reactions are optimized for generating maximum amounts of labeled DNA from 50 ng to 1 µg of DNA template. The reactions can be performed with denatured DNA derived from complete plasmid DNA or from gel-isolated restriction fragments of DNA. The reaction products range in size from less than 200 to greater than 2000 base pairs, depending upon the starting DNA fragment size.
Random primed probes are excellent for use in membrane hybridization applications and can be used in many circumstances for
in situ hybridization studies.
Standard hybridization and detection methods are used with chemically modified, random primed DNA probes.
A complete
BioProbe® Random Primed DNA Labeling System consists of the combination of two separate components: a Reagent Pack and one of two different Deoxynucleotide Packs.
The
BioProbe® Random Primed DNA Labeling System Reagent Pack contains all of the reagents (except deoxynucleotides) required to carry out 25 nonradioactive random primed DNA labeling reactions, including 10X hexanucleotide primers and Klenow DNA Polymerase. The Reagent Pack is completed by a vial of Stop Buffer and a vial of Control Template DNA. The various
BioProbe® Random Primed DNA Labeling System Deoxynucleotide Packs contain the modified deoxynucleotide mixed with the other deoxynucleoside triphosphates at an optimized concentration. A vial of Control DNA labeled with the analog nucleotide contained in the specific deoxynucleotide mixture completes the deoxynucleotide pack. The methods for labeling DNA with the
BioProbe® Random Primed DNA Labeling System are consistent for both of the haptens.
Product Specification
| Kit/Set Contains: | 10X Hexanucleotide Primers, 125 μl 10X concentrated in 10X Reaction Buffer Klenow DNA Polymerase, 50 μl 2 units/μl in storage buffer Stop Buffer, 125 μl 0.2 M EDTA Control Template DNA, 25 μl 10 ng/μl in TE Buffer (10mM Tris Buffer, pH 8.0, 1mM EDTA) |
| |
| Long Term Storage: | -20°C |
| |
| Background / Technical Information: | Enzo is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.For the Manual please click here. |
| |