Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

BIO-PROBE® Nick translation DNA labeling system reagent pack

Nick translation kit used in combination with deoxynucleotide packs for probe synthesis
 
ENZ-42710 25 Reactions 158.00 USD
Do you need bulk/larger quantities?
 
For FISH probe synthesis, it is recommended to use the Nick translation DNA labeling system (Prod. No. ENZ-42910).

Nick translation is recommended for labeling of double stranded DNA that is larger than 1kb. Nick translated probes are excellent for use in both in situ and membrane hybridization applications. Following hybridization with a nick translated probe carrying a chemical modification, the DNA probe can be detected by its interaction with an appropriate hapten-binding protein that is labeled with a fluorescent dye or a color producing enzyme.

The BIO-PROBE® Nick translation DNA labeling system reagent pack contains all of the reagents (except deoxynucleotides) required to carry out nick translation reactions, including DNase I and DNA Polymerase I.

The separately available deoxynucleotide packs are used in conjuction with the BIO-PROBE® Nick translation DNA labeling system reagent pack and are required for probe synthesis. The Bio-16-dUTP deoxynucleotide pack (Prod. No. ENZ-42712) is used for synthesizing biotin-labeled probes while the Fluorescein-12-dUTP deoxynucleotide pack (Prod. No. ENZ-42716) is used for synthesizing Fluorescein labeled probes.

The combination of BIO-PROBE® Nick translation DNA labeling system reagent pack and deoxynucleotide packs make a complete BIO-PROBE® Nick translation DNA labeling system that provides the researcher with a simple method for preparing a variety of labeled probes.

The complete BIO-PROBE® nick translation DNA labeling system is available as: BIO-PROBE® nick translation kit with Bio-16-dUTP (Prod. No. ENZ-42710-12) and the BIO-PROBE® Nick translation kit with Fluorescein-12-dUTP (Prod. No. ENZ-42710-16).

Product Specification

Applications:Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
 
Kit/Set Contains:DNase I, 50 µl 0.5 mg/ml in 0.1M MgCl2
50X DNase I Dilution Buffer, 1 ml 0.5 mg/ml BSA in 2.5M MgCl2
DNA Polymerase I, 125 µl 1 unit/µl in storage buffer
Stop Buffer, 125 µl 0.2M EDTA
Control Template DNA, 25 µl 0.2 µg/µl in TE Buffer (10mM Tris Buffer, pH 8.0, 1mM EDTA)
 
Use/Stability:After initial use, store the DNase I in 5-10µl aliquots at -20°C; avoid repeated freezing and thawing of the DNase I stock solution.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Technical Info/Product Notes:BIO-PROBE® is a trademark of Enzo Life Sciences, Inc. Several of Enzo's products and product applications are covered by US and foreign patents and patents pending.
 

Product Literature References

Limitations of in situ hybridization with total genomic DNA in routine screening for alien introgressions in wheat: A.J. Lukaszewski, et al.; Cytogenet. Genome Res. 109, 373 (2005), Abstract;
Genetic and physical mapping of homoeologous recombination points involving wheat chromosome 2B and rye chromosome 2R: A.J. Lukaszewski, et al.; Genome 47, 36 (2004), Abstract;

Related Products

Bio-16-dUTP deoxynucleotide pack for nick translation 

Print as PDF
 
ENZ-42712 25 Reactions 230.00 USD
Do you need bulk/larger quantities?
 

Fluorescein-12-dUTP deoxynucleotide pack for nick translation 

Print as PDF
 
ENZ-42716 25 Reactions 230.00 USD
Do you need bulk/larger quantities?
 

BIO-PROBE® Nick translation kit with bio-16-dUTP 

Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot | Print as PDF
 
ENZ-42710-12 25 Reactions 360.00 USD
Do you need bulk/larger quantities?
 

BIO-PROBE® Nick translation kit with fluorescein-12-dUTP 

Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot | Print as PDF
 
ENZ-42710-16 25 Reactions 355.00 USD
Do you need bulk/larger quantities?
 

Nick translation DNA labeling system 

Nick translation  system for preparing FISH probes
FISH | Print as PDF
 
ENZ-42910 50 Reactions 288.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By target:
DNA
By biological activity:
DNA Labeling kit
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

Brochures
Stem Cells
Stem Cells
Download as PDF

Catalogs
Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,