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BIO-PROBE® Nick translation kit with bio-16-dUTP

 
ENZ-42710-12 25 Reactions 360.00 USD
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For FISH probe synthesis, it is recommended to use the Nick translation DNA labeling system (Prod. No. ENZ-42910).

Nick translation is recommended for labeling of double stranded DNA that is larger than 1kb. Nick translated probes are excellent for use in both in situ and membrane hybridization applications. Following hybridization with a nick translated probe carrying a chemical modification, the DNA probe can be detected by its interaction with an appropriate hapten-binding protein that is labeled with a fluorescent dye or a color producing enzyme.

The BIO-PROBE® Nick Translation DNA Labeling System provides the researcher with a simple method for preparing a variety of hapten-labeled probes. A complete system consists of the combination of two separate components: a Reagent Pack and one of six different Deoxynucleotide Packs. The Reagent Pack contains all of the reagents (except deoxynucleotides) required to carry out nick translation reactions, including DNase I and DNA Polymerase I. The Nick Translation System Reagent Pack also contains a vial of concentrated DNase I Dilution Buffer, a vial of Stop Buffer for terminating the nick translation reactions and a vial of unlabeled Control Template DNA.

Product Specification

Applications:Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:BIO-PROBE® Nick Translation DNA Labeling System Reagent Pack (Prod. No. ENZ-42710)
Bio-16-dUTP Deoxynucleotide Pack for Nick Translation (Prod. No. ENZ-42712)
 

Product Literature References

Characterization of recombinants of the Aegilops peregrina-derived Lr59 translocation of common wheat: S.M. Pirseyedi, et al.; Theor. Appl. Genet. 128, 2403 (2015), Abstract;
Pivotal role of inosine triphosphate pyrophosphatase in maintaining genome stability and the prevention of apoptosis in human cells: M.R. Menezes, et al.; PLoS One 7, e32313 (2012), Abstract; Full Text

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