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CYTAG® TotalCGH Labeling kit

Complete kit to digest and label DNA for CGH+SNP arrays
 
ENZ-42674-0010 2x10 Reactions Inquire for pricing
 
ENZ-42674-0100 2x100 Reactions Inquire for pricing
Do you need bulk/larger quantities?
 
Also available in custom high throughput formats. 
  • Contains CYTAG® CGH Labeling kit, restriction enzymes AluI and RsaI, and PCR & Gel Clean-up Columns
  • Compatible with CGH and CGH+SNP arrays
  • Ability to perform total genomic DNA analysis without amplification or complexity reduction
  • Provides excellent dynamic analytical range for challenging and complex, heterogeneous samples
  • QC benchmarked and validated using high resolution arrays
  • Superior DLR scores (0.09-0.12), exceeding industry standards
Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations.
 
With optimized, proprietary reagents, Enzo's CYTAG® CGH Labeling Kits produce high quality data using as little as 0.25µg of genomic DNA, without a need for pre-amplification.

Bulk quantity kit, 2 x 1000 reactions, is available upon special request.
ENZ-42674-Kit Box
ENZ-42674 Comparative Analysis
Comparative analysis of chromosome 15. Upon scanning, the quality of the labeling in DNA samples was visually inspected to demonstrate detection of known deletion in the Prader-Willi DNA. This demonstrates the superior labeling obtained with the CGH labeling kit from Enzo Life Sciences when compared with a competitor’s kit.
ENZ-42674 Competitor
SNP analysis of a segment of chromosome 2. DNA was labeled with the Enzo kit (left) or a competitor’s kit (right). DNA was hybridized to Agilent’s CGH+SNP microarray. The Enzo kit cleanly identifies the sequence at SNPs while quantification of the SNP analysis of DNA labeled with a competitor’s labeling system is not as clean.
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ENZ-42674-Kit Box ENZ-42674 Comparative Analysis ENZ-42674 Competitor

Product Specification

Applications:CGH
 
Use/Stability:Store PCR & Gel Clean-up Columns at room temperature. All other components should be stored at -20°C. Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect Cyanine 3- and 5-dUTPs from light at all times. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:-0010 size:
ENZ-42671-K010
: Primers/Reaction Buffer, 1 x 400 µl; Cyanine 3-dUTP Nucleotide Mix, 2 x 50 µl; Cyanine 5-dUTP Nucleotide Mix, 2 x 50 µl; Klenow Exo-DNA Polymerase, 1 x 20 µl; Stop Buffer, 1 x 100 µl; Nuclease-free Water, 1 x 1 ml
ENZ-GEN100-0020: PCR & Gel Clean-up columns, 20 tests
ENZ-GEN108-1000: AluI, 1000 units, 10X Cutting Buffer
ENZ-GEN109-1000: RsaI, 1000 units, 10X Cutting Buffer

-0100 size:
ENZ-42671-K100: Primers/Reaction Buffer, 1 x 4 ml; Cyanine 3-dUTP Nucleotide Mix, 1 x 1 ml; Cyanine 5-dUTP Nucleotide Mix, 1 x 1 ml; Klenow Exo-DNA Polymerase, 1 x 0.2 ml; Stop Buffer, 1 x 1 ml; Nuclease-free Water, 1 x 10 ml
ENZ-GEN100-0200: PCR & Gel Clean-up columns, 200 tests
ENZ-GEN108-1000: AluI, 1000 units, 10X Cutting Buffer
ENZ-GEN109-1000: RsaI, 1000 units, 10X Cutting Buffer
 
Scientific Background:
 
Technical Info/Product Notes:Application Note:
Using CYTAG® TotalCGH Labeling Kit for CGH + SNP Array
 

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Superior labeling efficiency and better dye incorporation results in less failed runs
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BIOSCORE® Screening and amplification kit (20 reactions) 

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PCR & Gel Clean-up Kit 

Quick, easy kit for purifying DNA from PCR, gels and labeling reactions for a variety of downstream applications.
Print as PDF
 
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AluI 

Restriction  enzyme for molecular biology applications
Produced in an E. coli strain that carries the cloned AluI gene from Arthrobacter luteus., Southern Blot, Cloning, Genotyping, CGH | Print as PDF
 
ENZ-GEN108-1000 1000 U 82.00 USD
Do you need bulk/larger quantities?
 

RsaI 

Restriction  enzyme for molecular biology applications
Produced in an E. coli strain that carries the cloned RsaI gene from Rhodopseudomonas sphaeroides., Southern Blot, Cloning, Genotyping, CGH | Print as PDF
 
ENZ-GEN109-1000 1000 U 61.00 USD
Do you need bulk/larger quantities?
 

Human Cot DNA 

Blocks repetitive sequences to prevent nonspecific binding.
ISH (in situ hybridization), Microarray, Southern Blot, FISH, CGH | Print as PDF
 
ENZ-GEN116-0500 500 µg 200.00 USD
Do you need bulk/larger quantities?
 

Human Genomic DNA, Male 

A normal pool of male genomic DNA that can serve as a standard in molecular biology applications
PCR, Southern Blot, CGH | Print as PDF
 
ENZ-GEN117-0100 100 µg 160.00 USD
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Human Genomic DNA, Female 

A normal pool of female genomic DNA that can serve as a standard in molecular biology applications
PCR, Southern Blot, CGH | Print as PDF
 
ENZ-GEN118-0100 100 µg 160.00 USD
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