Contains CYTAG® CGH Labeling kit, restriction enzymes AluI and RsaI, and PCR & Gel Clean-up Columns
Compatible with CGH and CGH+SNP arrays
Ability to perform total genomic DNA analysis without amplification or complexity reduction
Provides excellent dynamic analytical range for challenging and complex, heterogeneous samples
QC benchmarked and validated using high resolution arrays
Superior DLR scores (0.09-0.12), exceeding industry standards
Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations.
With optimized, proprietary reagents, Enzo's CYTAG® CGH Labeling Kits produce high quality data using as little as 0.25µg of genomic DNA, without a need for pre-amplification.
Bulk quantity kit, 2 x 1000 reactions, is available upon special request.
SNP analysis of a segment of chromosome 2. DNA was labeled with the Enzo kit (left) or a competitor’s kit (right). DNA was hybridized to Agilent’s CGH+SNP microarray. The Enzo kit cleanly identifies the sequence at SNPs while quantification of the SNP analysis of DNA labeled with a competitor’s labeling system is not as clean.
Comparative analysis of chromosome 15. Upon scanning, the quality of the labeling in DNA samples was visually inspected to demonstrate detection of known deletion in the Prader-Willi DNA. This demonstrates the superior labeling obtained with the CGH labeling kit from Enzo Life Sciences when compared with a competitor’s kit.
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Product Details
Applications:
CGH
Use/Stability:
Store PCR & Gel Clean-up Columns at room temperature. All other components should be stored at -20°C. Stable for at least one year after receipt when stored as recommended.
Handling:
Protect Cyanine 3- and 5-dUTPs from light at all times. Avoid freeze/thaw cycles.
Shipping:
Dry Ice and Ambient Temperature
Long Term Storage:
-20°C
Contents:
-0010 size: ENZ-42671-K010: Primers/Reaction Buffer, 1 x 400 µl; Cyanine 3-dUTP Nucleotide Mix, 2 x 50 µl; Cyanine 5-dUTP Nucleotide Mix, 2 x 50 µl; Klenow Exo-DNA Polymerase, 1 x 20 µl; Stop Buffer, 1 x 100 µl; Nuclease-free Water, 1 x 1 ml ENZ-GEN100-0020: PCR & Gel Clean-up columns, 20 tests ENZ-GEN108-1000: AluI, 1000 units, 10X Cutting Buffer ENZ-GEN109-1000: RsaI, 1000 units, 10X Cutting Buffer
-0100 size: ENZ-42671-K100: Primers/Reaction Buffer, 1 x 4 ml; Cyanine 3-dUTP Nucleotide Mix, 1 x 1 ml; Cyanine 5-dUTP Nucleotide Mix, 1 x 1 ml; Klenow Exo-DNA Polymerase, 1 x 0.2 ml; Stop Buffer, 1 x 1 ml; Nuclease-free Water, 1 x 10 ml ENZ-GEN100-0200: PCR & Gel Clean-up columns, 200 tests ENZ-GEN108-1000: AluI, 1000 units, 10X Cutting Buffer ENZ-GEN109-1000: RsaI, 1000 units, 10X Cutting Buffer
Restriction enzyme for molecular biology applications
Produced in an E. coli strain that carries the cloned RsaI gene from Rhodopseudomonas sphaeroides., Southern Blot, Cloning, Genotyping, CGH | Print as PDF