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CYTAG® CGH Labeling kit

Superior labeling efficiency and better dye incorporation results in less failed runs
 
ENZ-42671-K010 2x10 Reactions Inquire for pricing
 
ENZ-42671-K100 2x100 Reactions Inquire for pricing
Do you need bulk/larger quantities?
 
Also available in custom high throughput formats. 
  • Ability to perform total genomic DNA analysis without amplification or complexity reduction
  • Provides excellent dynamic analytical range for challenging and complex, heterogeneous samples
  • QC benchmarked and validated using high resolution arrays
  • Superior DLR scores (0.09-0.12), exceeding industry standards
Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations.
 
With optimized, proprietary reagents, the Enzo Life Sciences CGH Labeling Kits for oligo arrays produce high quality data using as little as 0.25 µg of genomic DNA, without a need for pre-amplification.

Bulk quantity kit, 2 x 1000 reactions, is available upon special request.

For the purification of labeled DNA, use PCR & Gel Clean-up columns, Prod No. ENZ-GEN100.
Prader-Willi Syndr - Chr 15 sm 02
Analysis of syndromic DNA using an oligonucleotide microarray (Agilent 4 x 180K) demonstrated the characteristic deletion in 15q11.2-q13 (chromosome 15) found in patients with Prader-Willi syndrome.




ENZ-42671 Kit Box
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Prader-Willi Syndr - Chr 15 sm 02 ENZ-42671 Kit Box

Product Specification

Applications:CGH
 
Application Notes:Validated on Agilent SurePrint, OGT CytoSure™, and Illumina 24Sure Microarrays.
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect Cyanine 3- and 5-dUTPs from light at all times. Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:-K010 size: Primers/Reaction Buffer, 1 x 400 µL; Cyanine 3-dUTP Nucleotide Mix, 2 x 50 µL; Cyanine 5-dUTP Nucleotide Mix, 2 x 50 µL; Klenow DNA Polymerase, 1 x 20 µL; Stop Buffer, 1 x 100 µL; Nuclease-free Water, 1 x 1 mL
-K100 size: Primers/Reaction Buffer, 1 x 4 mL; Cyanine 3-dUTP Nucleotide Mix, 1 x 1 mL; Cyanine 5-dUTP Nucleotide Mix, 1 x 1 mL; Klenow DNA Polymerase, 1 x 0.2 mL; Stop Buffer, 1 x 1 mL; Nuclease-free Water, 1 x 10 mL
 
Scientific Background:
 
Technical Info/Product Notes:Application Notes:
Compatibility of CYTAG® CGH Labeling Kit from Enzo Life Sciences with SurePrint® G3 Human CGH 1x1M Microarrays from Agilent

Compatibility of CYTAG® CGH Labeling Kit with Illumina 24sure® Microarrays

CGH labeling protocol for small volumes of DNA sample

High Resolution Microarray Scanner used to Compare CGH Labeling Methods

Cited samples:
For an overview of Cited Samples by Sample Type, please click here.
For an overview of Cited Samples by Array Type, please click here.
 

Product Literature References

Comparison of clinical grade human platelet lysates for cultivation of mesenchymal stromal cells from bone marrow and adipose tissue: M. Juhl, et al.; Scand. J. Clin. Lab. Invest. 76, 93 (2016), Application(s): Labeling of DNA from bone marrow mesenchymal stem cells and adipose tissue-derived stromal cells, Abstract;
Induced pluripotent stem cell differentiation and three-dimensional tissue formation attenuate clonal epigenetic differences in trichohyalin: A. Petrova, et al.; Stem. Cells. Dev. 25, 1366 (2016), Application(s): Induced pluripotent stem cell lines, Abstract;
An interstitial de-novo microdeletion of 3q26.33q27.3 causing severe intrauterine growth retardation: A. Bouman, et al.; Clin. Dysmorphol. 24, 68 (2015), Abstract;
Breast cancers with a BRCA1-like DNA copy number profile recur less often than expected after high-dose alkylating chemotherapy: P.C. Schouten, et al.; Clin. Cancer Res. 21, 763 (2015), Application(s): Labeling of DNA extracted from FFPE breast cancer tissue, Abstract;
Clinical features associated with copy number variations of the 14q32 imprinted gene cluster: J.A. Rosenfeld, et al.; Am. J. Med. Genet. A. 167A, 345 (2015), Abstract;
Copy number profiling by array comparative genomic hybridization identifies frequently occurring BRCA2-like male breast cancer: H.D. Biesma, et al.; Genes Chromosomes Cancer 54, 734 (2015), Abstract;
Cross-platform assessment of genomic imbalance confirms the clinical relevance of genomic complexity and reveals loci with potential pathogenic roles in diffuse large B-cell lymphoma: L.M. Dias, et al.; Leuk. Lymphoma 16, 1 (2015), Abstract;
First contiguous gene deletion causing biotinidase deficiency: The enzyme deficiency in three Sri Lankan children: D.N. Senanayake, et al.; Mol. Gen. Met. Rep. 2, 81 (2015), Application(s): DNA Labeling,
Redefining the MED13L syndrome: A. Adegbola, et al.; Eur. J. Hum. Genet. 23, 1308 (2015), Abstract;
Three Huntington's disease specific mutation-carrying human embryonic stem cell lines have stable number of CAG repeats upon in vitro differentiation into cardiomyocytes: L. Jacquet, et al.; PLoS One 10, e0126860 (2015), Application(s): Labeling of DNA extracted from Huntington's disease human embryonic stem cells, Abstract; Full Text
Variant Rett syndrome in a girl with a pericentric X-chromosome inversion leading to epigenetic changes and overexpression of the MECP2 gene: J.P. Vieira, et al.; Int. J. Dev. Neurosci. 46, 82 (2015), Application(s): DNA Labeling, Abstract;
A new direction for prenatal chromosome microarray testing: software-targeting for detection of clinically significant chromosome imbalance without equivocal findings: J.W. Ahn, et al.; Peer J. 2, e354 (2014), Application(s): Labeling of DNA extracted from chorionic villi and amniotic fluid samples, Abstract; Full Text
Analysis of MYB oncogene in transformed adenoid cystic carcinomas reveals distinct pathways of tumor progression: A.F. Costa, et al.; Lab. Invest. 94, 692 (2014), Abstract;
Mutation screening and array comparative genomic hybridization using a 180K oligonucleotide array in VACTERL association: J. Winberg, et al.; PLoS One 9, e85313 (2014), Abstract; Full Text
Phenotypic features in patients with 15q11.2(BP1-BP2) deletion: Further delineation of an emerging syndrome: M. Cafferkey, et. al.; Am. J. Med. Genet. A. 164A, 1916 (2014), Abstract;
An improved method to extract DNA from 1ml of uncultured amniotic fluid from patients at less than 16 weeks’ gestation: A.L. Mosca-Boidron, et al.; PLoS One 8, e59956 (2013), Abstract; Full Text
Chromosome 20p11 gains are associated with liver-specific metastasis in patients with colorectal cancer: L.J. Mekenkamp, et al.; Gut 62, 94 (2013), Abstract;
DNA copy number profiling reveals extensive genomic loss in hereditary BRCA1 and BRCA2 ovarian carcinomas: M.M. Kamieniak, et al.; Br. J. Cancer 108, 1732 (2013), Abstract; Full Text
Focal aberrations indicate EYA2 and hsa‐miR‐375 as oncogene and tumor suppressor in cervical carcinogenesis: M. Bierkens, et al.; Genes Chromosomes Cancer 52, 56 (2013), Abstract;
Germline copy number variation of genes involved in chromatin remodelling in families suggestive of Li-Fraumeni syndrome with brain tumours: J. Aury-Landas, et al.; Eur. J. Hum. Genet. 21, 1369 (2013), Abstract;
Intellectual disability and hemizygous GPD2 mutation: D.Q. Barge-Schaapveld, et al.; Am. J. Med. Genet. A. 161A, 1044 (2013), Abstract;
Male-biased autosomal effect of 16p13.11 copy number variation in neurodevelopmental disorders: M. Tropeano, et al.; PLoS One 8, e61365 (2013), Application(s): Blood samples from patients with neurodevelopmental disorders, Abstract; Full Text
Novel candidate genes for 46,XY gonadal dysgenesis identified by a customized 1 M array-CGH platform: A. Norling, et al.; Eur. J. Med. Genet. 56, 661 (2013), Application(s): Blood from patients with disorder of sex development, Abstract;
Partial tetrasomy 14 associated with multiple malformations: J. Winberg, et al.; Am. J. Med. Genet. A. 161A, 1284 (2013), Abstract;
Polycomb silencing of the Drosophila 4E-BP gene regulates imaginal disc cell growth: H. Mason-Suares, et al.; Dev. Biol. 380, 111 (2013), Application(s): ChIP DNA samples, Abstract;
A rare microduplication in a familial case of annular pancreas and duodenal stenosis: E. Markjung, et al.; J. Pediatr. Surg. 47, 2039 (2012), Abstract;
CGH arrays compared for DNA isolated from formalin-fixed, paraffin-embedded material: O. Krijgsman, et al.; Genes Chromosomes Cancer 51, 344 (2012), Abstract;
Chromosome 5q loss in colorectal flat adenomas: Q.J. Voorham, et al.; Clin. Cancer Res. 18, 4560 (2012), Application(s): Colorectal flat adenoma, Abstract; Full Text
Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions: D. Ilic, et al.; Cytotherapy 14, 122 (2012), Application(s): Human embryonic stem cells, Abstract;
Differential copy number aberrations in novel candidate genes associated with progression from in situ to invasive ductal carcinoma of the breast: S. Liao, et al.; Genes Chromosomes Cancer 51, 1067 (2012), Application(s): FFPE of invasive ductal carcinoma of the breast, Abstract; Full Text
Endocrine and anatomical findings in a case of Solitary Median Maxillary Central Incisor Syndrome: K. Szakszon, et al.; Eur. J. Med. Genet. 55, 109 (2012), Abstract;
Exon-level array CGH in a large clinical cohort demonstrates increased sensitivity of diagnostic testing for Mendelian disorders: S. Aradhya, et al.; Genet. Med. 14, 594 (2012), Application(s): Labeling of DNA extracted from blood, Abstract; Full Text
Genomic aberrations relate early and advanced stage ovarian cancer: A. Zaal, et al.; Cell. Oncol. 35, 181 (2012), Application(s): Labeling of DNA from tumor tissue with cyanine 3-dUTP (Cy3) and cyanine 5-dUTP, Abstract; Full Text
A novel autosomal dominant condition consisting of congenital heart defects and low atrial rhythm maps to chromosome 9q: J.B. van de Meerakker, et al.; Eur. J. Hum. Genet. 19, 820 (2011), Abstract; Full Text
Deletion of chromosome 4q predicts outcome in stage II colon cancer patients: R.P. Brosens, et al.; Cell. Oncol. (Dordr) 34, 215 (2011), Abstract; Full Text
Detection of mosaicism for genome imbalance in a cohort of 3,042 clinical cases using an oligonucleotide array CGH platform: S. Hoang, et al.; Eur. J. Med. Genet. 54, 121 (2011), Application(s): Labeling of DNA from clinical blood samples, Abstract; Full Text
Genetic profile of adenoid cystic carcinomas (ACC) with high-grade transformation versus solid type: A.F. Costa, et al.; Cell. Oncol. (Dordr) 34, 369 (2011), Abstract;
Modeling familial Alzheimer's disease with induced pluripotent stem cells: T. Yagi, et al.; Hum. Mol. Genet. 20, 4530 (2011), Abstract; Full Text
The atypical 16p11.2 deletion: a not so atypical microdeletion syndrome?: D.Q. Barge-Schaapveld, et al.; Am. J. Med. Genet. A. 155A, 1066 (2011), Abstract;
The genomic relationship between primary breast carcinomas and their nodal metastases: M.M. Desouki, et al.; Cancer Invest. 29, 300 (2011), Abstract;
A triplication of the Williams-Beuren syndrome region in a patient with mental retardation, a severe expressive language delay, behavioural problems and dysmorphisms : G. Beunders, et al.; J. Med. Genet. 47, 271 (2010), Abstract;
Complete COL1A1 allele deletions in osteogenesis imperfecta: F.S. van Dijk, et al.; Genet. Med. 12, 736 (2010), Application(s): Samples from patients with osteogenesis imperfecta, Abstract;
Correlating array comparative genomic hybridization findings with histology and outcome in spitzoid melanocytic neoplasms: L. Ali, et al.; Int. J. Clin. Exp. Pathol. 3, 593 (2010), Abstract;
Intragenic deletions and a deep intronic mutation affecting pre-mRNA splicing in the dihydropyrimidine dehydrogenase gene as novel mechanisms causing 5-fluorouracil toxicity: A.B. van Kuilenburg, et al.; Hum. Genet. 128, 529 (2010), Application(s): Blood from patients with DPD deficiency, Abstract; Full Text
MLPA for confirmation of array CGH results and determination of inheritance: A. Hills, et al.; Mol. Cytogenet. 3, 19 (2010), Abstract;
The core genome of the anaerobic oral pathogenic bacterium Porphyromonas gingivalis: J. Brunner, et al.; BMC Microbiol. 10, 252 (2010), Application(s): Porphyromonas gingivalis, Abstract; Full Text
Validation and implementation of array comparative genomic hybridisation as a first line test in place of postnatal karyotyping for genome imbalance.: J.W. Ahn, et al.; Mol. Cytogenet. 3, 9 (2010), Abstract;
NMD inhibition fails to identify tumour suppressor genes in microsatellite stable gastric cancer cell lines: T.E. Buffart, et al.; BMC Med. Genomics 2, 39 (2009), Application(s): Gastric cancer cell lines, Abstract; Full Text
A comparative analysis of two tissue procurement approaches for the genomic profiling of clinical colorectal cancer samples: W.D. Mojica, et al.; Int. J. Colorectal Dis. 23, 1089 (2008), Application(s): Cells taken from patients with colorectal cancer, Abstract;
Across array comparative genomic hybridization: a strategy to reduce reference channel hybridizations: T.E. Buffart, et al.; Genes Chromosomes Cancer 47, 994 (2008), Abstract;
Genome-scale validation of deep-sequencing libraries: D. Schmidt, et al.; PLoS One 3, e3713 (2008), Abstract; Full Text
Germline rates of de novo meiotic deletions and duplications causing several genomic disorders: D.J. Tuner, et al.; Nat. Genet. 40, 90 (2008), Abstract; Full Text
NF1 microduplication first clinical report: association with mild mental retardation, early onset of baldness and dental enamel hypoplasia?: B. Grisart, et al.; Eur. J. Hum. Genet. 16, 305 (2008), Abstract; Full Text
Confirmation of a novel recurrent association: BCR-ABL t(9;22) and t(19;21): S. Struski, et al.; Cancer Genet. Cytogenet. 179, 127 (2007), Abstract;
Duplication of the entire 22.9 Mb human chromosome 21 syntenic region on mouse chromosome 16 causes cardiovascular and gastrointestinal abnormalities: Z. Li, et al.; Hum. Mol. Genet. 16, 1359 (2007), Abstract; Full Text
Gene-resolution analysis of DNA copy number variation using oligonucleotide expression microarrays: H. Auer, et al.; BMC Genomics 8, 111 (2007), Abstract; Full Text
Transcriptomic versus chromosomal prognostic markers and clinical outcome in uveal melanoma: L.A. Worley, et al.; Clin. Cancer Res. 13, 1466 (2007), Application(s): Primary uveal melanoma, Abstract; Full Text

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