CGH labeling kit for oligo arrays

Exceptional Cyanine Dye Performance with Proprietary Labeling Technology



ENZ-42671-K010	1 Kit	INQUIRE

ENZ-42671-K100	1 Kit	INQUIRE

Ability to perform total genomic DNA analysis without amplification or complexity reduction
Provides excellent dynamic analytical range for challenging and complex, heterogeneous samples
QC benchmarked and validated using high resolution arrays
Superior DLR scores (0.09-0.12), exceeding industry standards

Comparative genomic hybridization (aCGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. aCGH provides an understanding of genetic disorders, cancers and other genomic aberrations.

With optimized, proprietary reagents, the Enzo Life Sciences CGH Labeling Kits for oligo arrays produce high quality data using as little as 0.25 µg of genomic DNA, without a need for pre-amplification.

Bulk quantity kit, 2 x 1000 reactions, is available upon special request.

Analysis of syndromic DNA using an oligonucleotide microarray (Agilent 4 x 180K) demonstrated the characteristic deletion in 15q11.2-q13 (chromosome 15) found in patients with Prader-Willi syndrome.

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Product Specification

Quantity:	2 x 10 reactions or 2 x 100 reactions

Kit/Set Contains:	Kit 42671-K010: Primers/Reaction Buffer, 1 x 400 μl; Cyanine 3-dUTP Nucleotide Mix, 2 x 50 μl; Cyanine 5-dUTP Nucleotide Mix, 2 x 50 μl; Klenow Exo-DNA Polymerase, 1 x 20 μl; Stop Buffer, 1 x 100 μl; Nuclease-free Water, 1 x 1 ml Kit 42671-K100: Primers/Reaction Buffer, 1 x 4 ml; Cyanine 3-dUTP Nucleotide Mix, 1 x 1 ml; Cyanine 5-dUTP Nucleotide Mix, 1 x 1 ml; Klenow Exo-DNA Polymerase, 1 x 0.2 ml; Stop Buffer, 1 x 1 ml; Nuclease-free Water, 1 x 10 ml

Long Term Storage:	-20°C

Use/Stability:	Protect Cyanine 3- and 5-dUTPs from light at all times.

Handling:	Avoid freeze/thaw cycles.

Product Literature References

A triplication of the Williams-Beuren syndrome region in a patient with mental retardation, a severe expressive language delay, behavioural problems and dysmorphisms. : G. Beunders, et al.; J. Med. Genet. 47, 271 (2010), Abstract;

Correlating array comparative genomic hybridization findings with histology and outcome in spitzoid melanocytic neoplasms. : L. Ali, et al.; Int. J. Clin. Exp. Pathol. 3(6), 593 (2010), Abstract;

MLPA for confirmation of array CGH results and determination of inheritance. : A. Hills, et al.; Mol. Cytogenet. 3, 19 (2010), Abstract;

Validation and implementation of array comparative genomic hybridisation as a first line test in place of postnatal karyotyping for genome imbalance.: J.W. Ahn, et al.; Mol. Cytogenet. 3, 9 (2010), Abstract;