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BIOARRAY HIGHYIELD® RNA transcript labeling kit (T7)

The gold standard in transcript labeling, for use with Affymetrix® GeneChip® microarrays.
 
ENZ-42655-10 10 Reactions 845.00 USD
 
ENZ-42655-40 40 Reactions 2,645.00 USD
 
ENZ-42655-100 100 Reactions 4,955.00 USD
Do you need bulk/larger quantities?
 
  • Produces large amounts of RNA from template DNA
  • Incorporates biotin during transcription
The BIOARRAY HIGHYIELD® RNA Transcript Labeling System is the gold standard transcript labeling system and is intended for use with Affymetrix® GeneChip® brand eukaryotic expression microarrays.

The BIOARRAY HIGHYIELD® RNA Transcript Labeling Kit (T7) has been developed for the production of large amounts of hybridizable biotin-labeled RNA targets by in vitro transcription from bacteriophage T7 RNA polymerase promoters. Using T7 RNA polymerase and biotin-labeled nucleotides, large amounts of single stranded nonradioactive RNA molecules can be produced in vitro. Because of the nature of transcription reactions, many RNA copies of the template DNA are produced. Because RNA-DNA hybrids have a higher melting temperature than corresponding DNA-DNA hybrids, single-stranded RNA targets offer higher target avidity and greater sensitivity than DNA probes. RNA targets offer selectivity unavailable with DNA targets−being single stranded, they are strand specific and hybridize more effectively to probes because the target population does not self-hybridize.

Product Specification

Specificity:

Yield: Each lot is shown to generate 50 μg of cRNA when transcribed from a double stranded cDNA library generated from 2 μg total RNA (mixed tissue) in a single round of amplification.

Incorporation: Each lot is tested to ensure at least 10 % of incorporated nucleotides are biotin labeled.

RNase activity: Insignificant amounts of RNase (less than 100 fg RNase A activity in 1 μL of reagent) are detected.

% P value and 3’/5’ ratio: The cRNA synthesized by the kit is tested on Affymetrix HU133A 2.0 chip and shown to give > 35% P-calls, with 3’/5’ ratios for both β-actin and GAPDH of < 2.5.

 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:10X HY Reaction Buffer
10X Biotin-Labeled Ribonucleotides
10X DTT
10X RNase Inhibitor Mix
20X T7 RNA Polymerase
 

Product Literature References

STAT3-mediated upregulation of lncRNA HOXD-AS1 as a ceRNA facilitates liver cancer metastasis by regulating SOX4: H. Wang, et al.; Mol. Cancer 16, 136 (2017), Application(s): Microarray analysis, Abstract; Full Text
Alcohol-induced suppression of KDM6B dysregulates the mineralization potential in dental pulp stem cells: M. Hoang, et al.; Stem Cell Res. 17, 111 (2016), Application(s): Biotinylation of samples, Abstract;
Alterations of circulating lymphoid committed progenitor cellular metabolism after allogeneic stem cell transplantation in humans: S. Glauzy, et al.; Exp. Hematol. 44, 811 (2016), Application(s): RNA label - Peripheral blood cells, Abstract;
An immune-inflammation gene expression signature in prostate tumors of smokers: R.L. Prueitt, et al.; Cancer Res. 76, 1055 (2016), Abstract;
Biomarker evaluation of skeletal muscle toxicity following clofibrate administration in rats: K. Bodie, et al.; Exp. Toxicol. Pathol. 68, 289 (2016), Application(s): Synthesized cRNA from cDNA, Abstract;
Effects of intravitreal ranibizumab on the untreated eye and systemic gene expression profile in age-related macular degeneration: K. Michalska-Malecka, et al.; Clin. Interv. Aging. 11, 357 (2016), Abstract; Full Text
FoxP1 marks medium spiny neurons from precursors to maturity and is required for their differentiation: S.V. Precious, et al.; Exp. Neurol. 282, 9 (2016), Abstract;
GPR21 KO mice demonstrate no resistance to high fat diet induced obesity or improved glucose tolerance: J. Wang, et al.; F1000Res. 5, 136 (2016), Abstract; Full Text
Identification of a gene expression signature in peripheral blood of multiple sclerosis patients treated with disease-modifying therapies: C. Cordiglieri, et al.; Clin. Immunol. (2016), Application(s): Microarray gene profiling analysis, Abstract;
Identification of a RAI1-associated disease network through integration of exome sequencing, transcriptomics, and 3D genomics: M. N. Loviglio, et al.; Genome Med. 8, 105 (2016), Application(s): Microarray processing, Abstract; Full Text
Preclinical Evaluation of the Novel BTK Inhibitor Acalabrutinib in Canine Models of B-Cell Non-Hodgkin Lymphoma: B.K. Harrington, et al.; PLoS One 11, e0159607 (2016), Application(s): RNA extraction and gene expression profiling, Abstract; Full Text
Acute toxicity of CCl4 but not of paracetamol induces a transcriptomic signature of fibrosis in precision-cut liver slices: S. Vatakuti, et al.; Toxicol. In Vitro 29, 1012 (2015), Abstract;
Comparison of Drought Stress Response and Gene Expression between a GM Maize Variety and a Near-Isogenic Non-GM Variety: M. Gullì, et al.; PLoS One 10, e0117073 (2015), Application(s): In Vitro Transcription, Abstract; Full Text
Functional interaction between cyclooxygenase-2 and p53 in response to an endogenous electrophile: T. Kumagai, et al.; Redox Biol. 4, 74 (2015), Abstract; Full Text
Integrative genomic and transcriptomic characterization of matched primary and metastatic liver and colorectal carcinoma: S. Roessler, et al.; Int. J. Biol. Sci. 11, 88 (2015), Abstract; Full Text
KIM-1-mediated phagocytosis reduces acute injury to the kidney: L. Yang, et al.; J. Clin. Invest. 125, 1620 (2015), Abstract;
MDSC and TGFβ are required for facilitation of tumor growth in the lungs of mice exposed to carbon nanotubes: A.A. Shvedova, et al.; Cancer Res. 75, 1615 (2015), Abstract;
Mining cancer gene expression databases for latent information on intronic microRNAs: S. Monterisi, et al.; Mol. Oncol. 9, 473 (2015), Abstract;
Transcriptional changes in sensory ganglia associated with primary afferent axon collateral sprouting in spared dermatome model: B.J. Harrison, et al.; Genom. Data 6, 249 (2015), Application(s): In vitro transcription , Abstract;
Transcriptional profiling and biological pathway analysis of human equivalence PCB exposure in vitro: Indicator of disease and disorder development in humans: S. Ghosh, et al.; Environ. Res. 138C, 202 (2015), Application(s): Microarrays, Abstract;
Transforming Growth Factor beta in basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and keratoacanthoma (KA): M. Wyględowska-Kania, et al.; Postępy Nauk Medycznych 152 (2015), Application(s): Transcript Labeling, Full Text
Altered choroid plexus gene expression in major depressive disorder: C.A. Turner, et al.; Front Hum. Neurosci. 8, 238 (2014), Abstract; Full Text
Copy-neutral loss of heterozygosity is prevalent and a late event in the pathogenesis of FLT3/ITD AML: D.L. Stirewait, et al.; Blood Cancer J. 4, e208 (2014), Abstract; Full Text
Cytomegalovirus enhances macrophage TLR expression and MyD88-mediated signal transduction to potentiate inducible inflammatory responses: P.D. Smith, et al.; J. Immunol. 193, 5604 (2014), Abstract;
Divisional History and Hematopoietic Stem Cell Function during Homeostasis: J. Qiu, et al.; Stem Cell Reports 2, 473 (2014), Abstract; Full Text
Fibroblast-Like Synoviocytes Induce Calcium Mineral Formation and Deposition: Y. Sun, et al.; Arthritis 2014, Article ID 812678 (2014), Abstract; Full Text
Flies who cannot take the heat: genome‐wide gene expression analysis of temperature‐sensitive lethality in an inbred line of Drosophila melanogaster: C.J. Vermeulen, et al.; J. Evol. Biol. 27, 2152 (2014), Abstract;
Gene expression analysis in ovarian cancer–faults and hints from DNA microarray study: K.M. Lisowska, et al.; Front. Oncol. 4, 6 (2014), Abstract; Full Text
Gene expression signatures affected by alcohol-induced DNA methylomic deregulation in human embryonic stem cells: O. Khalid, et al.; Stem Cell Res. 12, 791 (2014), Abstract;
Genome-wide expression analysis in fibroblast cell lines from probands with Pallister Killian syndrome: M. Kaur, et al.; PLoS One 9, e108853 (2014), Abstract; Full Text
High CD49f expression is associated with osteosarcoma tumor progression: a study using patient‐derived primary cell cultures: P. Penfornis, et al.; Cancer Med. 3, 796 (2014), Abstract; Full Text
Identification of myelin transcription factor 1 (MyT1) as a subunit of the neural cell type-specific lysine-specific demethylase 1 (LSD1) complex: A. Yokoyama, et al.; J. Biol. Chem. 289, 18152 (2014), Abstract;
IL-17 induces an expanded range of downstream genes in reconstituted human epidermis model: A. Chiricozzi, et al.; PLoS One 9, e90284 (2014), Abstract; Full Text
N-octanoyl dopamine treatment of endothelial cells induces the unfolded protein response and results in hypometabolism and tolerance to hypothermia: E. Stamellou, et al.; PLoS One 9, e99298 (2014), Abstract; Full Text
Preventive effects of NSAIDs, NO-NSAIDs, and NSAIDs plus difluoromethylornithine in a chemically induced urinary bladder cancer model: H.L. Nicastro, et al.; Cancer Prev. Res. (Phila) 7, 246 (2014), Abstract;
Prosurvival function of the cellular apoptosis susceptibility/importin-α1 transport cycle is repressed by p53 in liver cancer: J. Winkler, et al.; Hepatology 60, 884 (2014), Abstract;
Regenerating 1 and 3b gene expression in the pancreas of type 2 diabetic Goto-Kakizaki (GK) rats: S. Calderari, et al.; PLoS One 9, e90045 (2014), Abstract; Full Text
Regulation of T lymphocyte activation by microRNA-21: L. Wang, et al.; Mol. Immunol. 59, 163 (2014), Abstract;
Staphylococcus aureus gene expression in a rat model of infective endocarditis: F. Hanses, et al.; Genome Med. 6, 93 (2014), Abstract; Full Text
Theiler's virus infection provokes the overexpression of genes coding for the chemokine Ip10 (CXCL10) in SJL/J murine astrocytes, which can be inhibited by modulators of estrogen receptors: N. Rubio, et al.; J. Neurovirol. 20, 485 (2014), Abstract;
Tissue remodeling in periplaque regions of multiple sclerosis spinal cord lesions: A. Lieury, et al.; Glia 62, 1645 (2014), Abstract;
VPA alleviates neurological deficits and restores gene expression in a mouse model of Rett syndrome: W. Guo, et al.; PLoS One 9, e100218 (2014), Abstract; Full Text
Tumourigenic characteristics of embryonal carcinoma cells as a model for studying tumour progression of human embryonic stem cells: S. Yang, et al.; Cell Prolif. 45, 299 (2012), Application(s): Comparison between human embryonic stem cells and embryonal carcinoma cells, Abstract;
Integrated genomic analysis of relapsed childhood acute lymphoblastic leukemia reveals therapeutic strategies: L.E. Hogan, et al.; Blood 118, 5218 (2011), Abstract; Full Text
In vitro and in vivo rationale for the triple combination of panobinostat (LBH589) and dexamethasone with either bortezomib or lenalidomide in multiple myeloma: E.M. Ocio, et al.; Haematologica 95, 794 (2010), Application(s): Multiple myeloma cell line (MM1S), Abstract; Full Text
Bone-marrow-derived side population cells for myocardial regeneration: H.A. Sadek, et al.; J. Cardiovasc. Transl. Res. 2, 173 (2009), Abstract;
Chemokine expression in melanoma metastases associated with CD8+ T-cell recruitment: H. Harlin, et al.; Cancer Res. 69, 3077 (2009), Application(s): Biopsies of melanoma metastases, Abstract; Full Text
Phenotypic subpopulations of metastatic colon cancer stem cells: genomic analysis: I.L. Botchkina, et al.; Cancer Genomics Proteomics 6, 19 (2009), Application(s): Human colonic adenocarcinoma cell line (HCT116), Abstract; Full Text
Selenium and vitamin E: cell type- and intervention-specific tissue effects in prostate cancer: D. Tsavachidou, et al.; J. Natl. Cancer Inst. 101, 306 (2009), Abstract; Full Text
The hematopoietic stem cell in chronic phase CML is characterized by a transcriptional profile resembling normal myeloid progenitor cells and reflecting loss of quiescence: I. Bruns, et al.; Leukemia 23, 892 (2009), Abstract;
Generation of superoxide anion in chloroplasts of Arabidopsis thaliana during active photosynthesis: a focus on rapidly induced genes: T.E. Scarpeci, et al.; Plant Mol. Biol. 66, 361 (2008), Application(s): Biotin-labelled cRNA synthesis from Arabidopsis thaliana samples, Abstract; Full Text
Genomic analysis of prostate cancer stem cells isolated from a highly metastatic cell line: R.A. Rowehl, et al.; Cancer Genomics Proteomics 5, 301 (2008), Application(s): Human prostate cancer cell line (PC3), Abstract; Full Text
Reduced immunogenicity of first-trimester human fetal pancreas: K. Brands, et al.; Diabetes 57, 627 (2008), Application(s): Human fetal pancreas, Abstract; Full Text
Tumor progression of culture-adapted human embryonic stem cells during long-term culture: S. Yang, et al.; Genes Chromosomes Cancer 47, 665 (2008), Abstract;
Heterogeneous expression of Wnt/beta-catenin target genes within colorectal cancer: F. Hlubek, et al.; Int. J. Cancer 121, 1941 (2007), Application(s): Biotin-labelled cRNA synthesis from human colorectal tissue samples, Abstract;
Gene expression profiles in rat intestine identify pathways for 1,25-dihydroxyvitamin D(3) stimulated calcium absorption and clarify its immunomodulatory properties: G.D. Kutuzova, et al.; Arch. Biochem. Biophys. 432, 152 (2004), Application(s): Biotin-labelled cRNA synthesis from mucosa rat samples, Abstract;

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