The BIOARRAY™ 3’-OH Terminal Labeling System is the gold standard end-labeling system for use with Affymetrix® DNA SNP, Resequencing and Prokaryotic microarrays.
The BIOARRAY™ Terminal Labeling Kit with Biotin-ddUTP has been developed for DNA probe array assays. This method uses Bio-ddUTP and terminal deoxynucleotide transferase to catalyze the addition of a single biotin-ddUMP to the 3’-OH terminus of an amplified and fragmented target DNA molecule. A standard reaction will label up to 100 picomoles (equivalent to 1µg of a 30-nucleotide sequence). When DNA fragments are labeled at the 3’-OH terminus, the sequence bias that occurs with either nick translation or random priming is eliminated.
Product Details
| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Contents: | Biotin-ddUTP (100X), 25 µl
5X Reaction Buffer, 500 µlPotassium cacodylate buffer, pH 7, containing β-mercaptoethanol and stabilizer
10X CoCl2 Solution, 250 µl
Terminal Deoxynucleotide Transferase (50X), 50 µl in storage buffer |
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| Regulatory Status: | RUO - Research Use Only |
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