Red 650 [Cyanine-5E] dUTP is a cyanine-5 derivative with enhanced spectral properties. This nucleotide analog can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3'-end labeling.
Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Red 650 dUTP can also be used for multicolor fluorescence labeling.
This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).
Product Details
Alternative Name:
Enhanced Cyanine 5-dUTP
Quantity:
Sufficient for approximately 49 reactions, following the recommended protocol of Prod. No. ENZ-GEN111.
Concentration:
1mM
Formulation:
Liquid. Solution in water.
Excitation maximum:
650 nm
Emission maximum:
662 nm
Extinction Coefficient:
250,000 M-1 cm-1 (650 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])
Correction Factor (260 nm):
0.05
Correction Factor (280 nm):
0.05
Purity:
≥93% (HPLC)
Purity Detail:
Purified by ion-exchange chromatography.
Appearance:
Blue liquid.
Applications:
FISH
Shipping:
Dry Ice
Long Term Storage:
-20°C
Use/Stability:
Stable for at least one year after receipt when stored as recommended.
Handling:
Protect from light. Avoid freeze/thaw cycles.
Technical Info/Product Notes:
Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
Regulatory Status:
RUO - Research Use Only
Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.
Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.
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Product Literature References
Calcineurin dephosphorylates topoisomerase IIβ and regulates the formation of neuronal-activity-induced DNA breaks: I.D. Ramirez, et al.; Mol. Cell 82, 3794 (2022), Abstract;
Hybridization-proximity labeling reveals spatially ordered interactions of nuclear RNA compartments: K. Yap, et al.; Mol. Cell 82, 463 (2022), Abstract;
Comprehensive chromosome end remodeling during programmed DNA elimination: J. Wang, et al.; Curr. Biol. 30, 3397 (2020), Abstract; Full Text
Highly Multiplexed Fluorescence in Situ Hybridization for in Situ Genomics: M.L. Onozato, et al.; J. Mol. Diagn. 21, 390 (2019), Application(s): Multiplex 2 and 3 colors in combination with Aqua 431-dUTP, Green 496-dUTP, Orange 552-dUTP, Gold 525-dUTP, Red 580-dUTP, Abstract; Full Text
Loss of p53 causes stochastic aberrant X-chromosome inactivation and female-specific neural tube defects: A.R.D. Delbridge, et al.; Cell Rep. 27, 442 (2019), Application(s): Generation of FISH probes, Abstract;
Structural and spatial chromatin features at developmental gene loci in human pluripotent stem cells: H. Ikeda, et al.; Nat. Commun. 8, 1616 (2017), Application(s): Generation of FISH probes, Abstract; Full Text
A portable BRCA1-HAC (human artificial chromosome) module for analysis of BRCA1 tumor suppressor function: A.V. Kononenko, et al.; Nucleic Acids Res. 42, e164 (2014), Abstract;
