Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Gold 550 dUTP

 
ENZ-42521 25 nmol 315.00 USD
Do you need bulk/larger quantities?
 
Gold 550  [Cyanine-3E] dUTP is a cyanine-3 derivative with enhanced spectral properties. This nucleotide analog can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Gold 550 dUTP can also be used for fluorescence labeling.

This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).

Product Specification

Alternative Name:Enhanced Cyanine 3-dUTP
 
Quantity:Sufficient for approximately 49 reactions, following the recommended protocol of Prod. No. ENZ-GEN111.
 
Concentration:1mM
 
Formulation:Liquid. Solution in water.
 
Absorbance maximum:550 nm
 
Emission maximum:564 nm
 
Extinction Coefficient:150,000 M-1 cm-1 (550 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])
 
Correction Factor (260 nm):0.08
 
Correction Factor (280 nm):0.08
 
Purity:≥93% (HPLC)
 
Purity Detail:Purified by ion-exchange chromatography.
 
Appearance:Dark pink liquid.
 
Applications:FISH
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
 
enz-42521
ENZ-42521 FC graph
Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.
ENZ-42521 FC table
Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.
Please mouse over
enz-42521 ENZ-42521 FC graph ENZ-42521 FC table

Related Products

Nick translation DNA labeling system 2.0 

Nick translation system for preparing FISH probes
ISH (in situ hybridization), FISH | Print as PDF
 
ENZ-GEN111-0050 50 tests 260.00 USD
Do you need bulk/larger quantities?
 

Nick translation DNA labeling system 

Nick translation  system for preparing FISH probes
FISH | Print as PDF
 
ENZ-42910 50 Reactions 288.00 USD
Do you need bulk/larger quantities?
 

Red 650 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42522 25 nmol 315.00 USD
Do you need bulk/larger quantities?
 

Green 496 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42831 25 nmol 250.00 USD
Do you need bulk/larger quantities?
 

Orange 552 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42842 25 nmol 300.00 USD
Do you need bulk/larger quantities?
 

Gold 525 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42843 25 nmol 318.00 USD
Do you need bulk/larger quantities?
 

Red 580 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42844 25 nmol 318.00 USD
Do you need bulk/larger quantities?
 

Green 500 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42845 25 nmol 250.00 USD
Do you need bulk/larger quantities?
 

Aqua 431 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42853 25 nmol 318.00 USD
Do you need bulk/larger quantities?
 

Human Cot DNA 

Blocks repetitive sequences to prevent nonspecific binding.
ISH (in situ hybridization), Microarray, Southern Blot, FISH, CGH | Print as PDF
 
ENZ-GEN116-0500 500 µg 200.00 USD
Do you need bulk/larger quantities?
 

Related Literature

Brochures
Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

Product Flyers
Label Your Own FISH Probes
Label Your Own FISH Probes
Download as PDF

Brochures
Stem Cells
Stem Cells
Download as PDF

Catalogs
Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,