Cyanine 5-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP.
Product Details
| Alternative Name: | Cyanine 5-uridine-5’-triphosphate (enhanced) |
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| Concentration: | 10mM |
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| Formulation: | Liquid. Solution in water. |
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| Excitation maximum: | 650 nm |
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| Emission maximum: | 665 nm |
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| Extinction Coefficient: | 250,000 M-1 cm-1 (650 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA]) |
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| Purity: | ≥93% (HPLC) |
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| Purity Detail: | Purified by ion-exchange chromatography. |
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| Appearance: | Blue liquid. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Technical Info/Product Notes: | Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending. |
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| Regulatory Status: | RUO - Research Use Only |
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