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BIOSCORE® Screening and amplification kit (20 reactions)

 
ENZ-42440 1 Kit 515.00 USD
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The BIOSCORE®  Screening and amplification kit is a rapid and reliable method to determine the quality of genomic DNA isolated from formalin-fixed paraffin-embedded (FFPE)  tissue samples before use in array-based genome-wide copy number analysis and other genomic profiling applications. This dual purpose kit utilizes a whole genome amplification (WGA) method to identify genomic DNA samples that are suitable for microarray analysis prior to labeling and to predict sample performance with virtually 100% concordance. After prescreening, the amplified genomic DNA can then be used in genomic profiling assays such as array analysis.

Product Specification

Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Contents:Primers, 400 µl
Enzyme, 20 µl
Stop Buffer, 110 µl
Nucleotide Mix, 200 µl
Nuclease-free Water, 1 ml
 

Product Literature References

A buffered alcohol-based fixative for histomorphologic and molecular applications: C. Perry, et al.; J. Histochem. Cytochem. 64, 425 (2016), Abstract;
A melanin-bleaching methodology for molecular and histopathological analysis of formalin-fixed paraffin-embedded tissue: J.Y. Chung, et al.; Lab Invest. 96, 1116 (2016), Application(s): DNA quality assessment, Abstract;
Genomic DNA copy number aberrations, histological diagnosis, oral subsite and aneuploidy in OPMDs/OSCCs: P. Castagnola, et al.; PLoS One 10, e0142294 (2015), Abstract; Full Text
Chromosome 20 aberrations at the diploid-aneuploid transition in sporadic colorectal cancer: M. Maffei, et al.; Cytogenet. Genome Res. 144, 9 (2014), Abstract;
Genomic patterns resembling BRCA1- and BRCA2-mutated breast cancers predict benefit of intensified carboplatin-based chemotherapy: M.A. Vollebergh, et al.; Breast Cancer Res. 16, R47 (2014), Abstract; Full Text
Focal aberrations indicate EYA2 and hsa-miR-375 as oncogene and tumor suppressor in cervical carcinogenesis: M. Bierkens, et al.; Genes Chromosomes Cancer 52, 56 (2013), Abstract;
A pressure cooking-based DNA extraction from archival formalin-fixed, paraffin-embedded tissue: J.Y. Chung, et al.; Anal. Biochem. 425, 128 (2012), Abstract; Full Text
Beyond KRAS mutation status: influence of KRAS copy number status and microRNAs on clinical outcome to cetuximab in metastatic colorectal cancer : L.J. Mekenkamp, et al.; BMC Cancer 12, 292 (2012), Abstract; Full Text
Differential copy number aberrations in novel candidate genes associated with progression from in situ to invasive ductal carcinoma of the breast: S. Liao, et al.; Genes Chromosomes Cancer 51, 1067 (2012), Abstract; Full Text
High-resolution copy number profiling by array CGH using DNA isolated from formalin-fixed, paraffin-embedded tissues: H.F. van Essen, et al.; Methods Mol. Biol. 838, 329 (2012), Abstract;
Molecular profiling reveals low-and high-grade forms of primary melanoma: K. Harbst, et al.; Clin. Cancer Res. 18, 4026 (2012), Abstract; Full Text
An aCGH classifier derived from BRCA1-mutated breast cancer and benefit of high-dose platinum-based chemotherapy in HER2-negative breast cancer patients: M.A. Vollebergh, et al.; Ann. Oncol. 22, 1561 (2011), Abstract; Full Text
Correlation between genomic alterations assessed by array comparative genomic hybridization, prognostically informative histologic subtype, stage, and patient : E. Rossi, et al.; Hum. Pathol. 42, 1937 (2011), Abstract;
Genomic changes in gliomas detected using single nucleotide polymorphism array in formalin-fixed, paraffin-embedded tissue: superior results compared with microsatellite analysis: S. Harada, et al.; J. Mol. Diagn. 13, 541 (2011), Abstract; Full Text
Genomic profiling of sinonasal squamous cell carcinoma: F. Lopez, et al.; Head Neck 33, 145 (2011), Abstract;
Laser capture microdissection (LCM) and whole genome amplification (WGA) of DNA from normal breast tissue --- optimization for genome wide array analyses: K.E. Aaltonen, et al.; BMC Res. Notes 4, 69 (2011), Abstract; Full Text
Correlating array comparative genomic hybridization findings with histology and outcome in spitzoid melanocytic neoplasms: L. Ali, et al.; Int. J. Clin. Exp. Pathol. 3, 593 (2010), Abstract; Full Text
Genomic aberrations in squamous cell lung carcinoma related to lymph node or distant metastasis: M.C. Boelens, et al.; Lung Cancer 66, 372 (2009), Abstract;
Methylation analysis by DNA immunoprecipitation (MeDIP): E.A. Vucic, et al.; Methods Mol. Biol. 556, 141 (2009), Abstract;
Across array comparative genomic hybridization: a strategy to reduce reference channel hybridizations: T.E. Buffart, et al.; Genes Chromosomes Cancer 47, 994 (2008), Abstract;
Challenges in array comparative genomic hybridization for the analysis of cancer samples: N.J. Nowak, et al.; Genet. Med. 9, 585 (2007), Abstract;
Detection of DNA copy number alterations in cancer by array comparative genomic hybridization: E. Michels, et al.; Genet. Med. 9, 574 (2007), Abstract;
DNA quality assessment for array CGH by isothermal whole genome amplification: T.E. Buffart, et al.; Cell. Oncol. 29, 351 (2007), Abstract;

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