100-Reaction single-round RNA amplification and biotin labeling system

Improved data quality and analysis results through greater biotin incorporation.



ENZ-42421-100	1 Pack	5'600.00 USD

Gold standard transcript labeling system maintains the value of legacy data
Incorporation of two biotin nucleotides yields brighter signal, improving data from microarray experiments
Convenient workflow with a flexible 4-16 hour transcription time and reagents supplied in a ready-to-use format.
Decreases experimental variation and standardizes biological data derived from microarrays with the universally accepted system.
Enables correlation of results from experiment-to-experiment, project -to-project and lab-to-lab.
Production of large amounts of biotin-labeled RNA targets by in vitro transcription available separately with our BioArray HighYield® RNA Transcript labeling

The Enzo Life Sciences' 100-Reaction Single-Round RNA Amplification and Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples (1µg-5µg) in less than 24 hours for array analysis. While cDNA quantities produced are sufficient for gene expression, manual sample preparation involving multiple enzymatic reactions and product purification steps are time consuming and error-prone. The 100-Reaction Single- Round RNA Amplification and Biotin Labeling System has been optimized for superior performance across several automated liquid handling platforms.

The result is a comprehensive system that reduces variability while subsequently improving reproducibility, data quality and throughput for automated genomic environments.

The complete system is composed of reagents for cDNA synthesis and in vitro transcription labeling and does not provide materials required for purification.

Product Specification

Quantity:	100 reactions

Quality Control:	Performance Testing: Biotin-labeled aRNA is generated from 500 ng of human reference total RNA following the procedures in the User Manual. Labeled aRNA is purified and yield determined by absorbance using a UV/Vis spectrophotometer. Determination of labeled aRNA transcript size is performed by capillary electrophoresis. Hybridized of aRNA to Affymetrix GeneChip HU133 2.0 Plus microarrays serves as a final functional test. Minimum Specifications: - aRNA yield must be > 23 μg. - aRNA purify must range between an A260/A280 ratio of 1.9 and 2.3. - aRNA size must be equal to or greater than 1200 nt. - Affymetrix GeneChip criteria: - % Present calls > than 40% - Scale factors < 3.0 - 5’/3’ ratios for actin and GAPDH ~ 1.0 ± 0.3

Kit/Set Contains:	400 μL, dNTP Mix (dN); 100 μL, Promoter Primer (P); 200 μL, First Strand Buffer (FB); 800 μL, DTT (D);100 μL, Reverse Transcriptase (RT); 100 μL, RNase Inhibitor (I); 500 μL, DNA Polymerase (DP); 100 μL, RNase H (RH); 1.5 mL, Second Strand Buffer (SB); 600 μL, IVT Reaction Buffer (RB); 600 µL, 10x Biotin-Labeled Ribonucleotides (B); 600 μL, Enhancer Cocktail (EC); 300 μL, T7 RNA Polymerase (T7); 13 mL, Nuclease-free Water (W)

Long Term Storage:	-20°C

Handling:	Avoid freeze/thaw cycles.

Background / Technical Information:	Technical Note: A Performance Comparison of the Enzo Single Round RNA Amplification and Biotin Labeling System. Best-in-Class performance: "Enzo kit is the best choice for routine Affymetrix GeneChip experiments."