Single-round RNA amplification and biotin labeling system



ENZ-42420-10	1 Pack	665.00 USD

Maintains the value of legacy data by the continued use of Enzo kit, the gold standard for GeneChip^® visualization.
Decreases experimental variation and standardizes biological data derived from microarrays with the universally accepted system.
Maximizes incorporation efficiency and produces strong, clear displays by utilizing two biotin-labeled nucleotides and a high quality T7 polymerase.
Convenient workflow with a flexible 4-16 hour transcription time and reagents supplied in a ready-to-use format.
Enables correlation of results from experiment-to-experiment, project-to-project and lab-to-lab.

The Single-Round RNA Amplification and Biotin Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis. This kit has been improved to yield sufficient aRNA quantity for most microarray platforms (10 µg minimum) with total RNA input of as low as 250 ng and as high as 5000 ng. The higher yield results from an improved cDNA purification system, now provided with the kit.

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Product Specification

Quantity:	10 reactions

Quality Control:	• aRNA yield is ≥ 23µg • aRNA purity range is between an A₂₆₀/A₂₈₀ ratio of 1.9 and 2.3 • aRNA size is equal to or greater than 1200 nt • Affymetrix GeneChip criteria: • % Present calls ≥ than 40% • Scale factors ≤ 3.0 • 5’/3’ ratios for actin and GAPDH ~ 1.0 ± 0.3

Kit/Set Contains:	Box A: 40 μL, dNTP Mix (dN); 10 μL, Promoter Primer (P); 20 μL, First Strand Buffer (FB); 80 μL, DTT (D); 10 μL, Reverse Transcriptase (RT); 10 μL, RNase Inhibitor (I); 50 μL, DNA Polymerase (DP); 10 μL, RNase H (RH); 150 μL, Second Strand Buffer (SB); 60 μL, IVT Reaction Buffer (RB); 60 µL, 10x Biotin-Labeled Ribonucleotides (B); 60 μL, Enhancer Cocktail (EC); 30 μL, T7 RNA Polymerase (T7); 2.5 mL, Nuclease-free Water (W) Box B: 4.5 mL, cDNA Binding Buffer (BB); 1.2 mL, 5X Wash Solution (WS); 10 cDNA Purification Columns; 10 cDNA Elution Tubes; 10 Collection Tubes

Long Term Storage:	-20°C

Use/Stability:	Store Box A at -20˚C in a non-frost free freezer. Store Box B at ambient condition.

Handling:	Avoid freeze/thaw cycles.

Background / Technical Information:	Technical Note: A Performance Comparison of the Enzo Single Round RNA Amplification and Biotin Labeling System. Best-in-Class performance: "Enzo kit is the best choice for routine Affymetrix GeneChip experiments." For the Manual please click here.