The Blur 8 BIO-PROBE® labeled probe is prepared by nick translation of a clone of the human alu repeat sequence. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).
Product Details
Concentration:
20µg/ml
Formulation:
Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA.
Applications:
Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
Application Notes:
The Blur 8 BIO-PROBE® labeled probe can serve as a positive control probe for human DNA sequences in both in situ and membrane hybridization applications. It can be used at a concentration of approximately 50ng/ml for Southern blots, Northern blots or dot blots. The probe can also be used for in situ hybridization applications at concentrations of approximately 0.5 to 5.0μg/ml. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes.The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes.
Shipping:
Dry Ice
Long Term Storage:
-20°C
Use/Stability:
Stable for one year after receipt when stored as recommended.
Technical Info/Product Notes:
BIO-PROBE® is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
Regulatory Status:
RUO - Research Use Only
Blur 8 BIOPROBE® staining in tonsil tissue. Blur 8 BIOPROBE® was hybridized to tonsil tissue. SIMPLY SENSITIVE® AP-NBT/BCIP in situ detection system (ENZ-32870) was used for visualization.
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Product Literature References
Diagnosis of human papillomavirus using in situ hybridization and in situ polymerase chain reaction: G.J. Nuovo.; Methods Mol. Biol. 179, 113 (2002), Abstract;
Squamous papilloma of the urinary tract is unrelated to condyloma acuminata: L. Cheng, et al.; Cancer 88, 1679 (2000), Abstract;
In situ viral DNA hybridization in diagnostic surgical pathology: W.W. Grody, et al.; Hum. Pathol. 18, 535 (1987), Abstract;
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes: D.J. Brigati, et al.; Virology 126, 32 (1983), Abstract;
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes: P.R. Langer, et al.; PNAS 78, 6633 (1981), Abstract;
