The BK Virus BIO-PROBE® labeled probe is prepared by nick translation of a clone of the entire BK virus genome. BK virus, a simian polyoma virus, is related to SV40 and also bears some similarity to the human virus JC virus. BK virus is highly tumorigenic in hamsters. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).
Product Details
Concentration:
20µg/ml
Formulation:
Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA.
Applications:
Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
Application Notes:
The BK Virus BIO-PROBE® labeled probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at concentrations of 50 to 100ng/ml. The probe can also be used for in situ hybridization applications at concentrations of 0.5 to 2.0μg/ml. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes. Complete kits and protocols are available for in situ hybridization and detection procedures (compatible with Enzo’s POLYVIEW® PLUS and SAVIEW® PLUS detection systems for IHC).
Shipping:
Dry Ice
Long Term Storage:
-20°C
Use/Stability:
Stable for one year after receipt when stored as recommended.
Technical Info/Product Notes:
BIO-PROBE® is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Polyomavirus large T antigen is prevalent in urothelial carcinoma post-kidney transplant: L. Yan, et al.; Hum. Pathol. 48, 122 (2016), Abstract;
Effect of steroid-free low concentration calcineurin inhibitor maintenance immunosuppression regimen on renal allograft histopathology and function: N. Nainani, et al.; Nephrol. Dial. Transplant. 27, 2077 (2012), Abstract; Full Text
Kidney allograft inflammation and fibrosis, causes and consequences: M. Gago, et al.; Am. J. Transplant. 12, 1199 (2012), Abstract; Full Text
Evaluation of human polyomavirus BK as a potential cause of villitis of unknown etiology and spontaneous abortion: M.M. Cajaiba, et al.; J. Med. Virol. 83, 1031 (2011), Abstract;
Collecting duct carcinoma arising in association with BK nephropathy post-transplantation in a pediatric patient. A case report with immunohistochemical and in situ hybridization study: L.L. Emerson, et al.; Pediatr. Transplant. 12, 600 (2008), Abstract;
Association of renal adenocarcinoma and BK virus nephropathy post transplantation: J.Y. Kausman, et al.; Pediatr. Nephrol. 19, 459 (2004), Abstract;
In situ viral DNA hybridization in diagnostic surgical pathology: W.W. Grody, et al.; Hum. Pathol. 18, 535 (1987), Abstract;
Construction and characterization of hybrid polyomavirus genomes: W.F. Chuke, et al.; J. Virol. 60, 960 (1986), Abstract;
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes: D.J. Brigati, et al.; Virology 126, 32 (1983), Abstract;
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes: P.R. Langer, et al.; PNAS 78, 6633 (1981), Abstract;
