The Lambda BIO-PROBE® labeled probe is prepared by nick translation of the lambda bacteriophage strain c1857ind1 Sam7.
Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).
Product Details
Concentration:
20µg/ml
Formulation:
Liquid. In 10mM TRIS-HCl, pH 7.5, containing 1mM EDTA.
Applications:
Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
Application Notes:
The BIO-PROBE® Lambda probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at concentrations of 50 to 100ng/ml. The probe can also be used as a negative control probe for in situ hybridization applications at concentrations of 0.5 to 2.0µg/ml.
The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes. Complete kits and protocols are available for in situ hybridization and detection procedures (Enzo’s SAVIEW® PLUS and POLYVIEW® PLUS enhanced in situ detection systems).
Shipping:
Dry Ice
Long Term Storage:
-20°C
Regulatory Status:
RUO - Research Use Only
General Literature References
In situ viral DNA hybridization in diagnostic surgical pathology: W.W. Grody, et al.; Hum. Pathol. 18, 535 (1987), Abstract;
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes: D.J. Brigati, et al.; Virology 126, 32 (1983), Abstract;
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes: P.R. Langer, et al.; PNAS 78, 6633 (1981), Abstract;
