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BIO-PROBE® Herpes simplex virus probe

 
ENZ-40838 2 µg 293.00 USD
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The Herpes Simplex Virus (HSV) BIO-PROBE® labeled probe is prepared by nick translation of two clones of HSV DNA, one 8 kb fragment from HSV 1 DNA and one 16 kb fragment from HSV 2 DNA. The probe is specific for herpes simplex viruses. It does not cross hybridize to cytomegalovirus nor Epstein-Barr virus, but is capable of detecting clinical isolates of HSV. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

Product Specification

Concentration:20µg/ml
 
Formulation:Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA.
 
Applications:Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
 
Application Notes:The Herpes Simplex Virus (HSV) BIO-PROBE® labeled probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at concentrations of 50 to 100ng/ml. The probe can also be used for in situ hybridization applications at concentrations of 0.5 to 1.0μg/ml. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes.The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes.Complete kits and protocols are available for in situ hybridization and detection procedures (Enzo’s SAVIEW® PLUS and POLYVIEW® PLUS in situ detection systems).
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for one year after receipt when stored as recommended.
 
Technical Info/Product Notes:BIO-PROBE® is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
 

Product Literature References

Importin-β and exportin-5 are indicators of acute viral infection: Correlation of their detection with commercially available detection kits: G.J. Nuovo, et al.; Ann. Diagn. Pathol. 34, 36 (2018), Application(s): ISH on vulvar lesions, Abstract;
Herpes simplex virus 1 infection induces activation and subsequent inhibition of the IFI16 and NLRP3 inflammasomes: K.E. Johnson, et al.; J. Virol. 87, 5005 (2013), Abstract; Full Text
Acute fatty liver of pregnancy-an underlying condition for herpes simplex type 2 fulminant hepatitis necessitating liver transplantation: B. Luzar, et al.; Z. Gastroenterol. 43, 451 (2005), Abstract;
Detection of herpes simplex virus DNA from genital lesions by in situ hybridization: A. Langenberg, et al.; J. Clin. Microbiol. 26, 933 (1988), Abstract;
In situ viral DNA hybridization in diagnostic surgical pathology: W.W. Grody, et al.; Hum. Pathol. 18, 535 (1987), Abstract;
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes: D.J. Brigati, et al.; Virology 126, 32 (1983), Abstract;
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes: P.R. Langer, et al.; PNAS 78, 6633 (1981), Abstract;

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