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BIO-PROBE® Herpes simplex virus probe

 
ENZ-40838 2 µg 281.00 USD
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The Herpes Simplex Virus (HSV) BIO-PROBE® labeled probe is prepared by nick translation of two clones of HSV DNA, one 8 kb fragment from HSV 1 DNA and one 16 kb fragment from HSV 2 DNA. The probe is specific for herpes simplex viruses. It does not cross hybridize to cytomegalovirus nor Epstein-Barr virus, but is capable of detecting clinical isolates of HSV. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

Product Specification

Concentration:20µg/ml
 
Formulation:Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA.
 
Applications:Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
 
Application Notes:The Herpes Simplex Virus (HSV) BIO-PROBE® labeled probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at concentrations of 50 to 100ng/ml. The probe can also be used for in situ hybridization applications at concentrations of 0.5 to 1.0μg/ml. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes.The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes.Complete kits and protocols are available for in situ hybridization and detection procedures (Enzo’s SIMPLY SENSITIVE® and ULTRA SENSITIVE® enhanced in situ detection systems).
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for one year after receipt when stored as recommended.
 
Technical Info/Product Notes:BIO-PROBE® is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
 

Product Literature References

Herpes simplex virus 1 infection induces activation and subsequent inhibition of the IFI16 and NLRP3 inflammasomes: K.E. Johnson, et al.; J. Virol. 87, 5005 (2013), Abstract; Full Text
Acute fatty liver of pregnancy-an underlying condition for herpes simplex type 2 fulminant hepatitis necessitating liver transplantation: B. Luzar, et al.; Z. Gastroenterol. 43, 451 (2005), Abstract;
Detection of herpes simplex virus DNA from genital lesions by in situ hybridization: A. Langenberg, et al.; J. Clin. Microbiol. 26, 933 (1988), Abstract;
In situ viral DNA hybridization in diagnostic surgical pathology: W.W. Grody, et al.; Hum. Pathol. 18, 535 (1987), Abstract;
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes: D.J. Brigati, et al.; Virology 126, 32 (1983), Abstract;
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes: P.R. Langer, et al.; PNAS 78, 6633 (1981), Abstract;

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