Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

BIO-PROBE® Cytomegalovirus probe

 
ENZ-40835 2 µg 281.00 USD
Do you need bulk/larger quantities?
 
The Cytomegalovirus (CMV) BIO-PROBE® labeled probe is prepared by nick translation of cloned fragments of the CMV Towne strain. The fragments include a total of 30-31 kb of DNA, approximately 20% of the CMV genome. The probe is specific for cytomegalovirus DNA. It does not hybridize to DNAs of other herpesviruses (i.e., herpes simplex virus or Epstein-Barr virus) but it has been found to hybridize to the DNA of CMV clinical isolates. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

Product Specification

Concentration:20µg/ml
 
Formulation:Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA.
 
Applications:Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
 
Application Notes:The Cytomegalovirus (CMV) BIO-PROBE® labeled probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at concentrations of 50 to 100ng/ml. The probe can also be used for in situ hybridization applications at concentrations of 0.5 to 1.0μg/ml. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for one year after receipt when stored as recommended.
 
Technical Info/Product Notes:BIO-PROBE® is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
 

Product Literature References

Detection of Epstein -Barr genome in the lymph nodes of Hodgkin's disease: C. Bellas, et al.; Postgrad. Med. J. 69, 916 (1993), Application(s): ISH used with CMV BioProbe as control to study lymp node tissue, Abstract; Full Text
Early and Rapid Diagnosis of CMV Infection by Nonradioactive in situ Hybridization in Pediatric Kidney Transplant Recipients: L. Murer, et al.; Nephron 60, 25 (1992), Application(s): ISH used to diagnose kidney cellular samples, Abstract;
In situ demonstration of Epstein-Barr virus in intravenous drug abusers with generalized lymphadenopathy: F. Mampaso, et al.; Postgrad. Med. J. 68, 739 (1992), Application(s): ISH used with CMV BioProbe as control to study lymp node tissue, Abstract; Full Text
Non-Hodgkin's lymphoma after prolonged remission of Hodgkin's disease in an HIV-infected patient: C. Montalban, et al.; Ann. Oncol. 8, 585 (1991), Abstract;
Cytomegalovirus and human herpesvirus-6 in sudden infant death syndrome: an in situ hybridization study: A. Coumbe, et al.; Pediatr. Pathol. 10, 483 (1990), Abstract;
Demonstration of cytomegalovirus retinitis by in situ DNA hybridization: D. Henderley, et al.; Retina 8, 177 (1988), Application(s): ISH of retinal tissue, Abstract;
Direct in situ hybridization for rapid detection of cytomegalovirus in bronchoalveolar lavage: L.H. Hilborne, et al.; Am. J. Clin. Pathol. 87, 766 (1987), Abstract;
In situ hybridization analysis of cytomegalovirus lytic infection in Kaposi's sarcoma associated with AIDS. A study of 14 autopsy cases: H. Hashimoto, et al.; Virchows Arch. A Pathol. Anat. Histopathol. 411, 441 (1987), Application(s): CMV assayed with ISH for paraffin embedded samples, Abstract;
In situ viral DNA hybridization in diagnostic surgical pathology: W.W. Grody, et al.; Hum. Pathol. 18, 535 (1987), Abstract;
Viral Diagnosis by in situ Hybridization: Description of a Rapid Simplified Colorimetric Method: E. Unger, et al.; Am. J. Surg. Pathol. 10, 1 (1986), Application(s): Colorimetric in situ DNA hybridization to diagnose viral infections, Abstract;
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes: D.J. Brigati, et al.; Virology 126, 32 (1983), Abstract;
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes: P.R. Langer, et al.; PNAS 78, 6633 (1981), Abstract;

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis

RELATED PRODUCTS

By target:
DNA
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

Catalogs
Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

Product Flyers
Antiviral Signaling
Antiviral Signaling
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,