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BIO-PROBE® Cytomegalovirus probe

 
ENZ-40835 2 µg 291.00 USD
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The Cytomegalovirus (CMV) BIO-PROBE® labeled probe is prepared by nick translation of cloned fragments of the CMV Towne strain. The fragments include a total of 30-31 kb of DNA, approximately 20% of the CMV genome. The probe is specific for cytomegalovirus DNA. It does not hybridize to DNAs of other herpesviruses (i.e., herpes simplex virus or Epstein-Barr virus) but it has been found to hybridize to the DNA of CMV clinical isolates. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

Product Specification

Concentration:20µg/ml
 
Formulation:Liquid. In 10mM TRIS HCl, pH 7.5, containing 1mM EDTA.
 
Applications:Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot
 
Application Notes:The Cytomegalovirus (CMV) BIO-PROBE® labeled probe can be used in a variety of hybridization techniques, including Southern blots, Northern blots or dot blots, at concentrations of 50 to 100ng/ml. The probe can also be used for in situ hybridization applications at concentrations of 0.5 to 1.0μg/ml. Biotinylated probes have been shown to hybridize to homologous DNA at the same rate and to the same extent as non-biotinylated probes. The hybridized biotinylated DNA probe can be detected by its interaction with biotin-binding proteins, such as avidin, streptavidin or antibodies coupled to fluorescent dyes or color producing enzymes.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for one year after receipt when stored as recommended.
 
Technical Info/Product Notes:BIO-PROBE® is a trademark of Enzo Life Sciences, Inc. Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
 

Product Literature References

Detection of Epstein -Barr genome in the lymph nodes of Hodgkin's disease: C. Bellas, et al.; Postgrad. Med. J. 69, 916 (1993), Application(s): ISH used with CMV BioProbe as control to study lymp node tissue, Abstract; Full Text
Early and Rapid Diagnosis of CMV Infection by Nonradioactive in situ Hybridization in Pediatric Kidney Transplant Recipients: L. Murer, et al.; Nephron 60, 25 (1992), Application(s): ISH used to diagnose kidney cellular samples, Abstract;
In situ demonstration of Epstein-Barr virus in intravenous drug abusers with generalized lymphadenopathy: F. Mampaso, et al.; Postgrad. Med. J. 68, 739 (1992), Application(s): ISH used with CMV BioProbe as control to study lymp node tissue, Abstract; Full Text
Non-Hodgkin's lymphoma after prolonged remission of Hodgkin's disease in an HIV-infected patient: C. Montalban, et al.; Ann. Oncol. 8, 585 (1991), Abstract;
Cytomegalovirus and human herpesvirus-6 in sudden infant death syndrome: an in situ hybridization study: A. Coumbe, et al.; Pediatr. Pathol. 10, 483 (1990), Abstract;
Demonstration of cytomegalovirus retinitis by in situ DNA hybridization: D. Henderley, et al.; Retina 8, 177 (1988), Application(s): ISH of retinal tissue, Abstract;
Direct in situ hybridization for rapid detection of cytomegalovirus in bronchoalveolar lavage: L.H. Hilborne, et al.; Am. J. Clin. Pathol. 87, 766 (1987), Abstract;
In situ hybridization analysis of cytomegalovirus lytic infection in Kaposi's sarcoma associated with AIDS. A study of 14 autopsy cases: H. Hashimoto, et al.; Virchows Arch. A Pathol. Anat. Histopathol. 411, 441 (1987), Application(s): CMV assayed with ISH for paraffin embedded samples, Abstract;
In situ viral DNA hybridization in diagnostic surgical pathology: W.W. Grody, et al.; Hum. Pathol. 18, 535 (1987), Abstract;
Viral Diagnosis by in situ Hybridization: Description of a Rapid Simplified Colorimetric Method: E. Unger, et al.; Am. J. Surg. Pathol. 10, 1 (1986), Application(s): Colorimetric in situ DNA hybridization to diagnose viral infections, Abstract;
Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes: D.J. Brigati, et al.; Virology 126, 32 (1983), Abstract;
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes: P.R. Langer, et al.; PNAS 78, 6633 (1981), Abstract;

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