Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

PATHO-GENE® AP-NBT/BCIP HPV in situ typing assay (types 6/11, 16/18 and 31/33/51)

Complete assay for in situ hybridization typing of HPV 6/11, 16/18, and 31/33/51
 
ENZ-32895 10 assays 1,403.00 USD
Do you need bulk/larger quantities?
 
The PATHO-GENE® human papillomavirus (HPV) in situ typing assay for tissue sections provides reagents and materials for (a) preparation and pretreatment and (b) hybridization/detection and typing of HPV DNA (using alkaline phosphatase-NBT/BCIP for signal generation) in 10 tissue biopsy specimens. This product is for research use only and is not to be used for diagnostic procedures.

The PATHO-GENE® human papillomavirus (HPV) in situ typing assay for tissue sections employs separate mixtures of HPV-specific probes to identify HPV types 6/11, 16/18, or 31/33/51. HPV types 6 and 11 are primarily associated with benign lesions presenting as condylomas and mild dysplasia. HPV types 16 and 18 are more commonly associated with cervical intraepithelial neoplasia (CIN) and carcinoma in situ (CIS). HPV types 31, 33 or 51 can be associated with either condyloma or CIN and CIS, presumably dependent upon other, as yet undefined, contributing factors or agents. The PATHO-GENE® human papillomavirus (HPV) in situ typing assay for tissue sections can be used to detect and identify HPV DNA in tissue sections under conditions that preserve cellular morphology.

Product Specification

Applications:Colorimetric detection
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Kit/Set Contains:HPV 6/11 Probe Mix, 0.5 ml (pink) Mixture of biotin-labeled HPV 6 and HPV 11-specific probes in buffered formamide and hybridization enhancers
HPV 16/18 Probe Mix, 0.5 ml (purple) Mixture of biotin-labeled HPV 16 and HPV 18-specific probes in buffered formamide and hybridization enhancers
HPV 31/33/51 Probe Mix, 0.5 ml (green) Mixture of biotin-labeled HPV 31, HPV 33 and HPV 51-specific probes in buffered formamide and hybridization enhancers
Hybridization Wash Reagent, 15 ml (white) Buffered formamide
AP Detection Reagent, 15 ml (orange) Streptavidin-biotinylated alkaline phosphatase in buffered saline with stabilizers and preservative
NBT Reagent, 0.4 ml (amber) Nitroblue tetrazolium in solvent
BCIP Reagent, 0.4 ml (amber) Bromochloroindolyl phosphate in solvent
AP Reaction Buffer, 2 x 10 ml (gray) Buffered saline and alkaline phosphatase enhancers
Counterstain Concentrate, 30 ml Eosin Y (certified) in alcohol
Proteinase K, 5 mg Lyophilized powder
SIGNASURE® Wash Buffer Salts, 4 packets Each packet yields 1 liter of buffer
Pretreated Specimen Slides, 20 slides10 single-well plus 10 double-well slides for mounting formalin-fixed paraffin-embedded tissue specimens

SOLD SEPARATELY: HPV 16 Probe Control Slide (Prod. No. ENZ-31877), Double-well slide with fixed HPV 16-positive cells
 
Technical Info/Product Notes:This product or the use of this product is covered by one or more Enzo patents, including, but not limited to, the following: U.S. Patent No. 4,994,373 and Canadian Patent No. 1,309,672; and patents pending.
 

Product Literature References

The utility of p16 immunostaining in fine needle aspiration in p16 positive head and neck squamous cell carcinoma: B. Xu, et al.; Hum. Pathol. 54, 193 (2016), Application(s): CISH using probes against high-risk HPV types, Abstract;
Enhanced expression of PD L1 in cervical intraepithelial neoplasia and cervical cancers: L. Mezache, et al.; Mod. Pathol. 28, 1594 (2015), Application(s): Determination of HPV in human tissue, Abstract;
Strong inverse correlation between microRNA-125b and human papillomavirus DNA in productive infection: G.J. Nuovo, et al.; Diagn. Mol. Pathol. 19, 135 (2010), Abstract; Full Text
In situ determination of T-cell receptor beta expression patterns: G.J. Nuovo, et al.; J. Histochem. Cytochem. 49, 139 (2001), Abstract;
Buschke-Loewenstein tumour infiltrating pelvic organs: A. Grassegger, et al.; Br. J. Dermatol. 130, 221 (1994), Application(s): in situ hybridization, Abstract;
Human papillomavirus detection in genital lesions by in situ hybridization and ultrastructural observations: L.H. Oliveira, et al.; Mem. Inst. Oswaldo Cruz. 89, 195 (1994), Application(s): in situ hybridization, Abstract; Full Text
Histological diagnosis of human papillomavirus (HPV)-related dysplasia: quality control by in situ hybridization (ISH) analysis: L. Cadorin, et al.; Eur. J. Gynaecol. Oncol. 13, 427 (1992), Application(s): in situ hybridization, Abstract;
In situ hybridization for the detection of human papillomavirus (HPV) in gynaecological biopsies. A study of two commercial kits: I. Zehbe, et al.; Anticancer Res. 12, 1383 (1992), Application(s): in situ hybridization, Abstract;
Laryngeal papillomatosis with human papillomavirus DNA contracted by a laser surgeon: P. Hallmo & O. Naess; Eur. Arch. Otorhinolaryngol. 248, 425 (1991), Application(s): in situ hybridization, Abstract; Full Text
Detection of human papillomavirus in nongenital Bowen's disease by in situ DNA hybridization: A.H. Kettler, et al.; Arch. Dermatol. 126, 777 (1990), Application(s): in situ hybridization, Abstract;

Related Products

PATHO-GENE® HRP-AEC HPV in situ typing assay (types 6/11, 16/18 and 31/33/51) 

Colorimetric detection | Print as PDF
 
ENZ-32877 20 assays 2,550.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® AP-NBT/BCIP HPV in situ screening assay 

Colorimetric detection | Print as PDF
 
ENZ-32879 20 assays 1,403.00 USD
Do you need bulk/larger quantities?
 

HPV 16 control slide 

Print as PDF
 
ENZ-31877 1 Slide 56.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® HPV type 16/18/31/33/51 probe 

HPV probe that can be optimized for use with polymer detection system or used as optimized RTU with Enzo's Streptavidin detection methods.
Print as PDF
 
ENZ-32882-6000 6 ml Inquire for pricing
 
ENZ-32882-1000 1 ml 648.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® HPV screening probe 

Print as PDF
 
ENZ-32884-6000 6 ml Inquire for pricing
 
ENZ-32884 1 ml 648.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® HPV type 6/11 probe 

A mixture of biotin-labeled HPV 6 and HPV 11-specific probes for in situ hybridization
Print as PDF
 
ENZ-32885-6000 6 ml Inquire for pricing
 
ENZ-32885 1 ml 852.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® HPV type 16/18 probe 

A mixture of biotin-labeled HPV 16 and HPV 18-specific probes for in situ hybridization
Print as PDF
 
ENZ-32886-6000 6 ml Inquire for pricing
 
ENZ-32886 1 ml 541.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® HPV type 31/33/51 probe 

A mixture of biotin-labeled HPV 31, HPV 33 and HPV 51-specific probes for in situ hybridization
Print as PDF
 
ENZ-32887-6000 6 ml Inquire for pricing
 
ENZ-32887 1 ml 541.00 USD
Do you need bulk/larger quantities?
 

In situ hybridization wash reagent 

Print as PDF
 
ENZ-33809 30 ml 112.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By target:
Papillomavirus
By biological activity:
Papillomavirus Detection kit
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

Catalogs
Genomics & Molecular Biology
Genomics & Molecular Biology
Download as PDF

Brochures
Innovative Products to Analyze Immunological Disorders
Innovative Products to Analyze Immunological Disorders
Download as PDF

Brochures
Human Papillomavirus - Clear Results, Innovative Solutions for HPV Detection
Human Papillomavirus - Clear Results, Innovative Solutions for HPV Detection
Download as PDF

Product Flyers
Antiviral Signaling
Antiviral Signaling
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,