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PATHO-GENE® AP-NBT/BCIP HPV in situ screening assay

 
ENZ-32879 20 assays 1,403.00 USD
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The PATHO-GENE® Human papillomavirus (HPV) in situ screening assay for tissue sections provides reagents and materials for (a) preparation and pretreatment and (b) hybridization/ detection and typing of HPV DNA (using alkaline phosphatase-NBT/BCIP for signal generation) in 20 tissue biopsy specimens. This product is for research use only and is not to be used for diagnostic procedures.

The PATHO-GENE® Human papillomavirus (HPV) in situ screening assay for tissue sections employs a mixture of HPV-specific probes that have been specially formulated to detect most HPV types that have been associated with anogenital papillomas. Among the HPV types detected with this assay system are those commonly associated with benign lesions presenting as condylomas and mild dysplasia, as well as types commonly associated with cervical intraepithelial neoplasia (CIN) and carcinoma in situ (CIS). The PATHO-GENE® Human papillomavirus (HPV) in situ screening assay for tissue sections can be used to detect HPV DNA in tissue sections under conditions that preserve cellular morphology.

Product Specification

Applications:Colorimetric detection
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Kit/Set Contains:HPV DNA Probe Mix, 1 ml (small, white) Mixture of biotin-labeled HPV-specific DNA probes in buffered formamide and hybridization enhancers
Hybridization Wash Reagent, 15 ml (large, white) Buffered formamide
AP Detection Reagent, 15 ml (orange) Streptavidin-biotinylated alkaline phosphatase in buffered saline with stabilizers and preservative
NBT Reagent, 0.4 ml (amber) Nitroblue tetrazolium in solvent
BCIP Reagent, 0.4 ml (amber) Bromochloroindolyl phosphate in solvent
AP Reaction Buffer, 4 x 5 ml (gray) Buffered saline and alkaline phosphatase enhancers
Counterstain Concentrate, 30 ml Eosin Y (certified) in alcohol
Proteinase K, 5 mg Lyophilized powder
SIGNASURE® Wash Buffer Salts, 4 packets Each packet yields 1 liter of buffer
Pretreated Specimen Slides, 20 slides 20 single-well slides for mounting formalin-fixed paraffin-embedded tissue specimens

SOLD SEPARATELY: HPV Control Slide, Double-well slide with fixed HPV 16-positive cells
 
Technical Info/Product Notes:This product or the use of this product is covered by one or more Enzo patents, including, but not limited to, the following: U.S. Patent No. 4,994,373 and Canadian Patent No. 1,309,672; and patents pending.
 

Product Literature References

Different miRNA signatures of oral and pharyngeal squamous cell carcinomas: a prospective translational study: C.B. Lajer, et al.; Br. J. Cancer 104, 830 (2011), Abstract; Full Text
Detection and sequences of human papillomavirus DNA in nongenital seborrhoeic keratosis of immunopotent individuals: A. Gushi, et al.; J. Dermatol. Sci. 31, 143 (2003), Abstract;
Detection of human papillomavirus type 60 in plantar cysts and verruca plantaris by the in situ hybridization method using digoxigenin labeled probes: M. Kawase, et al. ; J. Dermatol. 21, 709 (1994), Application(s): in situ hybridization, Abstract;

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PATHO-GENE® AP-NBT/BCIP HPV in situ typing assay (types 6/11, 16/18 and 31/33/51) 

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For Research Use Only. Not for use in diagnostic procedures.
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