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SIMPLY SENSITIVE® Hrp-DAB in situ detection system

ENZ-32840 20 assays 145.00 USD
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The SIMPLY SENSITIVE® Horseradish Peroxidase-DAB In Situ Detection System is an easy-to-use system designed for use in the detection of biotin-labeled nucleic acids following in situ hybridization of biotin-labeled nucleic acid probes to fixed cells and tissues, or following in situ labeling of target nucleic acids in fixed cells and tissues with biotin-labeled nucleotides. The kit is optimized for use on routinely processed (formalin-fixed and paraffin-embedded) tissues, as well as tissue cryosections and cultured cells. The SIMPLY SENSITIVE® Horseradish Peroxidase-DAB In Situ Detection System provides a sensitive in situ detection system for biotinlabeled nucleic acids that takes advantage of the tight binding of biotin by streptavidin. Detection is accomplished in two steps. First, a streptavidin-horseradish peroxidase complex is bound to the biotin of the nucleic acid probe or the biotin label of the target nucleic acid. In the second step, the entire complex is visualized after conversion of the substrate and chromogen (hydrogen peroxide and diaminobenzidine) into a localized brown precipitate. Following counterstaining, the cells are observed by light microscopy.

Product Specification

Applications:Colorimetric detection
Handling:Store the Blue Counterstain at room temperature away from direct bright light. Store the DAB Reagent at -20°C.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
Contents:Detection Reagent, 7 ml (yellow) Streptavidin-horseradish peroxidase complex in phosphate buffered saline and stabilizers
DAB Reagent, 1 ml (brown) Diaminobenzidine in buffered stabilizers
Substrate Reagent, 2 x 6 ml (gray) Hydrogen peroxide in acetate buffer
Blue Counterstain, 7 ml (blue) Ready-to-use aqueous-based hematoxylin
Mixing Bottle, 1 vial (gray) 3 ml empty vial for use in preparing up to 3 ml of Color Reaction Mixture
ENZO Wash Buffer Salts, 2 packets Each packet yields one liter of Wash Buffer (phosphate buffered saline)
Technical Info/Product Notes:In nucleic acid assays, this product or its use are covered by one or more claims of Enzo patents, including, but not limited to the following: U.S. Patent No. 4,994,373; Canadian Patent No. 1,309,672; and patents pending.

Product Literature References

Molecular mechanisms of cell death in periventricular leukomalacia: H. Kadhim, et al.; Neurology 67, 293 (2006), Abstract;
A non-invasive technique for studying oral epithelial Epstein–Barr virus infection and disease: D.M. Wailing, et al.; Oral Oncol. 39, 436 (2003), Abstract;
Apoptosis in various organs of preterm infants: histopathologic study of lung, kidney, liver, and brain of ventilated infants: B. Hargitai, et al.; Pediatr. Res. 50, 110 (2001), Abstract;
C2-ceramide induces apoptosis in a human squamous cell carcinoma cell line: H. Sugiki, et al.; Br. J. Dermatol. 143, 1154 (2000), Abstract;
Better-surviving liver grafts by the injection of anti-CD2 antibody: the important roles of host CD8+ and CD2+ CD28+ T cells in chronic graft rejection and beta type platelet-derived growth factor receptor (PDGFR-beta) expression on apoptotic liver grafts: T. Nakatsuji, et al.; Tohoku J. Exp. Med. 187, 215 (1999), Abstract; Full Text
Prostatic intraepithelial neoplasia and apoptosis in benign prostatic hyperplasia before and after the Chernobyl accident in Ukraine: A.F. Vosianov, et al.; Pathol. Oncol. Res. 5, 28 (1999), Abstract; Full Text

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