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SIMPLY SENSITIVE® Hrp-AEC in situ detection system

 
ENZ-32830 20 assays 145.00 USD
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The SIMPLY SENSITIVE® Horseradish Peroxidase-AEC In Situ Detection System is an easy-to-use system designed for use in the detection of biotin-labeled nucleic acids following in situ hybridization of biotin-labeled nucleic acid probes to fixed cells and tissues, or following in situ labeling of target nucleic acids in fixed cells and tissues with biotin-labeled nucleotides. The kit is optimized for use on routinely processed (formalin-fixed and paraffin-embedded) tissues, as well as tissue cryosections and cultured cells. The SIMPLY SENSITIVE® Horseradish Peroxidase-AEC In Situ Detection System provides a sensitive in situ detection system for biotin-labeled nucleic acids that takes advantage of the tight binding of biotin by streptavidin. Detection is accomplished in two steps. First, a streptavidin-horseradish peroxidase complex is bound to the biotin of the nucleic acid probe or the biotin label of the target nucleic acid. In the second step, the entire complex is visualized after conversion of the substrate and chromogen (hydrogen peroxide and aminoethylcarbazole) into a localized brick red precipitate. Following counterstaining, the cells are observed by light microscopy.

Product Specification

Applications:Colorimetric detection
 
Handling:Store the Blue Counterstain at room temperature away from direct bright light. Store the AEC Reagent at -20°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Kit/Set Contains:Detection Reagent, 7 ml (yellow) Streptavidin-horseradish peroxidase complex in phosphate buffered saline and stabilizers
AEC Reagent, 1 ml (red) Aminoethylcarbazole in dimethylformamide
Reaction Buffer/Substrate Reagent, 2 x 6 ml (gray) Hydrogen peroxide in acetate buffer and reaction enhancers
Blue Counterstain, 7 ml (blue) Ready-to-use aqueous-based hematoxylin
Mixing Bottle, 1 vial (gray) 3 ml empty vial for use in preparing up to 3 ml of Color Reaction Mixture
ENZO Wash Buffer Salts, 2 packets Each packet yields one liter of Wash Buffer (phosphate buffered saline)
 
Technical Info/Product Notes:In nucleic acid assays, this product or its use are covered by one or more claims of Enzo patents, including, but not limited to the following: U.S. Patent No. 4,994,373; Canadian Patent No. 1,309,672; and patents pending.
 

Product Literature References

Amphipathic DNA polymers exhibit antiherpetic activity in vitro and in vivo: D.I. Bernstein, et al.; Antimicrob. Agents Chemother. 52, 2727 (2008), Abstract; Full Text
Comparative activities of lipid esters of cidofovir and cyclic cidofovir against replication of herpesviruses in vitro: S.L. Williams-Aziz, et al.; Antimicrob. Agents Chemother. 49, 3724 (2005), Abstract; Full Text
Inhibition of herpesvirus replication by a series of 4-oxo-dihydroquinolines with viral polymerase activity: C.B. Hartline, et al.; Antiviral Res. 65, 97 (2005), Abstract;
Determination of antiviral efficacy against lymphotropic herpesviruses utilizing flow cytometry: M.C. Long, et al.; Antiviral Res. 58, 149 (2003), Abstract;
In vitro activities of benzimidazole D- and L-ribonucleosides against herpesviruses: S.L. Williams, et al.; Antimicrob. Agents Chemother. 47, 2186 (2003), Abstract; Full Text
In vitro activities of methylenecyclopropane analogues of nucleosides and their phosphoralaninate prodrugs against cytomegalovirus and other herpesvirus infections: R.J. Rybak, et al.; Antimicrob. Agents Chemother. 44, 1506 (2000), Abstract; Full Text

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