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NGAL (human) ELISA kit

Highly senstitive ELISA, measuring as little as 4 pg/ml of NGAL
 
BPD-KIT-036 96 wells 689.00 USD
Do you need bulk/larger quantities?
 
  • Fast results from up to 40 samples in duplicate in just 4 hours per 96 well plate
  • Highly sensitive measurement, detecting as low as 4 pg/ml of NGAL
  • Ready-to-use pre-coated plate and individual calibrators save time and reduce errors
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
A colorimetric immunometric ELISA kit for the measurement of NGAL in human samples. Absorbance is read at 450nm.
BPD-KIT-036 std curve
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BPD-KIT-036 std curve

Product Specification

Alternative Name:Neutrophil gelatinase-associated lipocalin, Lipocalin 2, Siderocalin, Oncogene 24p3
 
Sensitivity:4pg/ml (range 10-1000pg/ml)
 
Assay Time:<4 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of NGAL in human urine, plasma, serum, tissue extracts, and culture supernatants.
 
Species reactivity:Human
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:1: 12 strips of 8 Microwells each coated with anti-NGAL (96 wells) + frame 2: Sample Diluent 3: NGAL Calibrators 1-8; 0, 10, 25, 50, 100, 250, 500, 1000 pg/mL 4: 25X Wash Solution Concentrate 5: Biotinylated NGAL Antibody 6: HRP-Streptavidin Conjugate 7: TMB Substrate 8: Stop Solution
 
Scientific Background:NGAL (also known as Lipocalin-2 or Neutrophil Gelatinase-associated Lipocalin) is a small, ~25kDa secreted protein expressed in epithelial tissues throughout the body.
 
Technical Info/Product Notes:Please click here for the original manufacturer datasheet.
 
UniProt ID:P80188
 
Manufacturer:Manufactured by BioPorto Diagnostics.
 

Product Literature References

An AKI biomarker lipocalin 2 in the blood derives from the kidney in renal injury but from neutrophils in normal and infected conditions: J. Kanda, et al.; Clin. Exp. Nephrol. 19, 99 (2015), Abstract;
Urinary neutrophil gelatinase-associated lipocalin predicts kidney outcome and death in patients with cirrhosis and bacterial infections: R. Barreto, et al.; J. Hepatol. 61, 35 (2014), Application(s): ELISA using human urine, Abstract;
A comparison of the analytical performance of five commercially available assays for neutrophil gelatinase-associated lipocalin using urine: R.L. Kift, et al.; Ann. Clin. Biochem. 50, 236 (2013), Abstract;
Albumin oxidation leads to neutrophil activation in vitro and inaccurate measurement of serum albumin in patients with diabetic nephropathy: R. Michelis, et al.; Free Radic. Biol. Med. 60, 49 (2013), Abstract;
Association between plasma neutrophil gelatinase associated lipocalin level and obstructive sleep apnea or nocturnal intermittent hypoxia: K. Murase, et al.; PLoS One 8, e54184 (2013), Abstract;
BK virus-associated nephropathy: neutrophil gelatinase-associated lipocalin as a new diagnostic tool?: S. Rau, et al.; Clin. Transplant. 27, E184 (2013), Abstract;
Can neutrophil gelatinase-associated lipocalin help depict early contrast material-induced nephropathy?: A. Lacquaniti, et al.; Radiology 267, 86 (2013), Abstract;
Circulating levels of neutrophil gelatinase-associated lipocalin (NGAL) correlate with the presence and severity of preeclampsia: S.M. Kim, et al.; Reprod. Sci. 20, 1083 (2013), Abstract;
Day-to-day variation of urinary NGAL and rational for creatinine correction: J. Helmersson-Karlqvist, et al.; Clin. Biochem. 46, 70 (2013), Abstract;
Differential expression of cancer-associated proteins in breastmilk: W. Qin, et al.; Breastfeed Med. 8, 120 (2013), Abstract;
Discovery and validation of cell cycle arrest biomarkers in human acute kidney injury: K. Kashani, et al.; Crit. Care 17, R25 (2013), Abstract;
Impact of severe sepsis on serum and urinary biomarkers of acute kidney injury in critically ill children: an observational study: M. Di Nardo, et al.; Blood Purif. 35, 172 (2013), Abstract;
Prognostic value of serum neutrophil gelatinase-associated lipocalin in metastatic and nonmetastatic colorectal cancer: J. Martí, et al.; World J. Surg. 37, 1103 (2013), Abstract;
Prophylactic perioperative sodium bicarbonate to prevent acute kidney injury following open heart surgery: a multicenter double-blinded randomized controlled trial: M. Haase, et al.; PLoS Med. 10, e1001426 (2013), Abstract;
Serum neutrophil gelatinase-associated lipocalin as a predictor of the development of bronchopulmonary dysplasia in preterm infants: H. Inoue, et al.; Early Hum. Dev. 89, 425 (2013), Abstract;
The clinical utility of plasma neutrophil gelatinase-associated lipocalin in acute kidney injury: J.W. Pickering, et al.; Blood Purif. 35, 295 (2013), Abstract;
The role of urinary liver-type fatty acid-binding protein in critically ill patients: E. Cho, et al.; J. Korean Med. Sci. 28, 100 (2013), Abstract;
Urine Biomarkers Neutrophil Gelatinase-Associated Lipocalin (NGAL) and Kidney Injury Molecule-1 (KIM-1) Have Different Patterns in Heart Failure Exacerbation: M. Park, et al.; Biomark. Insights 8, 15 (2013), Abstract;
Albuminuria increases cystatin C excretion: implications for urinary biomarkers: M. Nejat, et al.; Nephrol. Dial. Transplant. 27 Suppl 3, iii96 (2012), Abstract;
Are serum and urine neutrophil gelatinase-associated lipocalin predictive of renal graft function in short term?: N. Rahimzadeh, et al.; Pediatr. Transplant. 16, 796 (2012), Abstract;
Association between systemic neutrophil gelatinase-associated lipocalin and anemia, relative hypochromia, and inflammation in chronic systolic heart failure: K. Shrestha, et al.; Congest. Heart Fail. 18, 239 (2012), Abstract;
Delayed graft function (DGF) after living donor kidney transplantation: a study of possible explanatory factors: J. Salamzadeh, et al.; Ann. Transplant. 17, 69 (2012), Abstract;
Evaluation of the impact of shock wave lithotripsy on kidneys using a new marker: how do neutrophil gelatinese-associated lypocalin values change after shock wave lithotripsy?: F. Zekey, et al.; Urology 80, 267 (2012), Abstract;
Failure of remote ischemic preconditioning to reduce the risk of postoperative acute kidney injury in children undergoing operation for complex congenital heart disease: a randomized single-center study: K.R. Pedersen, et al.; J. Thorac. Cardiovasc. Surg. 143, 576 (2012), Abstract;
Increased plasma levels of NGAL, a marker of neutrophil activation, in patients with abdominal aortic aneurysm: P. Ramos-Mozo, et al.; Atherosclerosis 220, 552 (2012), Abstract;
Mild elevation of urinary biomarkers in prerenal acute kidney injury: K. Doi, et al.; Kidney Int. 82, 1114 (2012), Abstract;
Neutrophil gelatinase-associated lipocalin as an early biomarker of acute kidney injury in liver transplantation: T.D. Jeong, et al.; Clin. Transplant. 26, 775 (2012), Abstract;
Renal function and solitary kidney disease: Wilms tumour survivors versus patients with unilateral renal agenesis: J. Stefanowicz, et al.; Kidney Blood Press. Res. 35, 174 (2012), Abstract;
Serum levels of gelatinase associated lipocalin as indicator of the inflammatory status in coronary artery disease: N. Kafkas, et al.; Int. J. Inflam. 2012, 189797 (2012), Abstract;
Spironolactone diminishes urinary albumin excretion in patients with type 1 diabetes and microalbuminuria: a randomized placebo-controlled crossover study: S.E. Nielsen, et al.; Diabet. Med. 29, e184 (2012), Abstract;
The effect of RAAS blockade on markers of renal tubular damage in diabetic nephropathy: u-NGAL, u-KIM1 and u-LFABP: S.E. Nielsen, et al.; Scand J. Clin. Lab Invest. 72, 137 (2012), Abstract;
Urinary markers of kidney injury and kidney function decline in HIV-infected women: M.G. Shlipak, et al.; J. Acquir. Immune Defic. Syndr. 61, 565 (2012), Abstract;
Urinary neutrophil gelatinase-associated lipocalin accurately detects acute allograft rejection among other causes of acute kidney injury in renal allograft recipients: N. Heyne, et al.; Transplantation 93, 1252 (2012), Abstract;
Urinary NT-proBNP, NGAL, and H-FABP may predict hemodynamic relevance of patent ductus arteriosus in very low birth weight infants: V. Tosse, et al.; Neonatology 101, 260 (2012), Abstract;
Clinical outcome of renal tubular damage in chronic heart failure: K. Damman, et al.; Eur. Heart J. 32, 2705 (2011), Abstract;
Improved performance of urinary biomarkers of acute kidney injury in the critically ill by stratification for injury duration and baseline renal function: Z.H. Endre, et al.; Kidney Int. 79, 1119 (2011), Abstract;
Kidney injury molecule-1 and N-acetyl-β-D-glucosaminidase in chronic heart failure: possible biomarkers of cardiorenal syndrome: C.G. Jungbauer, et al.; Eur. J. Heart. Fail. 13, 1104 (2011), Abstract;
KIM-1 and NGAL: new markers of obstructive nephropathy: A. Wasilewska, et al.; Pediatr. Nephrol. 26, 579 (2011), Abstract;
Molecular MRI of murine atherosclerotic plaque targeting NGAL: a protein associated with unstable human plaque characteristics: B.C. te Boekhorst, et al.; Cardiovasc. Res. 89, 680 (2011), Abstract;
Neutrophil gelatinase-associated lipocalin at ICU admission predicts for acute kidney injury in adult patients: H.R. H. de Geus, et al.; Am. J. Respir. Crit. Care Med. 183, 907 (2011), Abstract;
Neutrophil gelatinase-associated lipocalin concentrations predict development of acute kidney injury in neonates and children after cardiopulmonary bypass: C.D. Krawczeski, et al.; J. Pediatr. 158, 1009 (2011), Abstract;
Renal dysfunction is a stronger determinant of systemic neutrophil gelatinase-associated lipocalin levels than myocardial dysfunction in systolic heart failure: K. Shrestha, et al.; J. Card. Fail. 17, 472 (2011), Abstract;
Serum neutrophil gelatinase-associated lipocalin - a sensitive novel marker of renal impairment in liver cirrhosis?: A.L. Gerbes, et al.; Digestion 84, 82 (2011), Abstract;
Temporal relationship and predictive value of urinary acute kidney injury biomarkers after pediatric cardiopulmonary bypass: C.D. Krawczeski, et al.; J. Am. Coll. Cardiol. 58, 2301 (2011), Abstract;
Tubular markers do not predict the decline in glomerular filtration rate in type 1 diabetic patients with overt nephropathy: S.E. Nielsen, et al.; Kidney Int. 79, 1113 (2011), Abstract;
Urinary neutrophil gelatinase-associated lipocalin and progression of diabetic nephropathy in type 1 diabetic patients in a four-year follow-up study: S.E. Nielsen, et al.; Nephron Clin. Pract. 118, c130 (2011), Abstract;
Urinary neutrophil gelatinase-associated lipocalin as a marker of acute kidney injury after orthotopic liver transplantation: G. Wagener, et al.; Nephrol. Dial. Transplant. 26, 1717 (2011), Abstract;
Urinary neutrophil gelatinase-associated lipocalin at birth predicts early renal function in very low birth weight infants: G. La Manna, et al.; Pediatr. Res. 70, 379 (2011), Abstract;
Volume status and diuretic therapy in systolic heart failure and the detection of early abnormalities in renal and tubular function: K. Damman, et al.; J. Am. Coll. Cardiol. 57, 2233 (2011), Abstract;
Bile duct ligation in developing rats: temporal progression of liver, kidney, and brain damage: J.M. Sheen, et al.; J. Pediatr. Surg. 45, 1650 (2010), Abstract;
Neutrophil Gelatinase-Associated Lipocalin (NGAL) and Kidney Injury Molecule 1 (KIM1) in patients with diabetic nephropathy: a cross-sectional study and the effects of lisinopril: S.E. Nielsen, et al.; Diabet. Med. 27, 1144 (2010), Abstract;
Neutrophil gelatinase-associated lipocalin (NGAL): validation of commercially available ELISA: K.R. Pedersen, et al.; Scand J. Clin. Lab Invest. 70, 374 (2010), Abstract;
Pathological significance of a panel of urinary biomarkers in patients with drug-induced tubulointerstitial nephritis: Y. Wu, et al.; Clin. J. Am. Soc. Nephrol. 5, 1954 (2010), Abstract;
Possible relationship between neutrophil gelatinase-associated lipocalin, hepcidin, and inflammation in haemodialysed patients: J. Malyszko, et al.; Nephron Clin. Pract. 115, c268 (2010), Abstract;
Safety of low volume iodinated contrast administration for arteriovenous fistula intervention in chronic kidney disease stage 4 or 5 utilizing a bicarbonate prophylaxis strategy: E. Eisenhart, et al.; Semin. Dial. 23, 638 (2010), Abstract;
Serum neutrophil gelatinase-associated lipocalin correlates with kidney function in heart allograft recipients: P. Przybylowski, et al.; Transplant Proc. 42, 1797 (2010), Abstract;
Urinary neutrophil gelatinase-associated lipocalin as a novel biomarker for disease activity in lupus nephritis: T. Rubinstein, et al.; Rheumatology (Oxford) 49, 960 (2010), Abstract;
Urinary neutrophil gelatinase-associated lipocalin: A potential biomarker for predicting rapid progression of drug-induced chronic tubulointerstitial nephritis: Y. Wu, et al.; Am. J. Med. Sci. 339, 537 (2010), Abstract;
Urinary neutrophil-gelatinase associated lipocalin is a potential noninvasive marker for renal scarring in patients with vesicoureteral reflux: M. Ichino, et al.; J. Urol. 183, 2001 (2010), Abstract;
Urine neutrophil gelatinase-associated lipocalin: an independent predictor of adverse outcomes in acute kidney injury: H.N. Yang, et al.; Am. J. Nephrol. 31, 501 (2010), Abstract;
Implication of granulocyte-macrophage colony-stimulating factor induced neutrophil gelatinase-associated lipocalin in pathogenesis of rheumatoid arthritis revealed by proteome analysis: M. Katano, et al.; Arthritis Res. Ther. 11, R3 (2009), Abstract;
NGAL: a new missing link between inflammation and uremic anemia?: D. Bolignano, et al.; Ren. Fail. 31, 622 (2009), Abstract;
Comparison of the effects of gelatin and a modern hydroxyethyl starch solution on renal function and inflammatory response in elderly cardiac surgery patients: J. Boldt, et al.; Br. J. Anaesth. 100, 457 (2008), Abstract;
Effect of a single intravenous immunoglobulin infusion on neutrophil gelatinase-associated lipocalin levels in proteinuric patients with normal renal function: D. Bolignano, et al.; J. Investig. Med. 56, 997 (2008), Abstract;
Increased incidence of acute kidney injury with aprotinin use during cardiac surgery detected with urinary NGAL: G. Wagener, et al.; Am. J. Nephrol. 28, 576 (2008), Abstract;
Neutrophil gelatinase-associated lipocalin as a biomarker of disease activity in pediatric lupus nephritis: M. Suzuki, et al.; Pediatr. Nephrol. 23, 403 (2008), Abstract;
Neutrophil gelatinase-associated lipocalin expression in chronic myeloid leukemia: C. Villalva, et al.; Leuk. Lymphoma 49, 984 (2008), Abstract;
Neutrophil gelatinase-associated lipocalin reflects the severity of renal impairment in subjects affected by chronic kidney disease: D. Bolignano, et al.; Kidney Blood Press. Res. 31, 255 (2008), Abstract;
NGAL (neutrophil gelatinase-associated lipocalin) and cystatin C: are they good predictors of contrast nephropathy after percutaneous coronary interventions in patients with stable angina and normal serum creatinine?: H. Bachorzewska-Gajewska, et al.; Int. J. Cardiol. 127, 290 (2008), Abstract;
Serum neutrophil gelatinase-associated lipocalin as a marker of renal function in hypertensive and normotensive patients with coronary artery disease: J. Malyszko, et al.; Nephrology (Carlton) 13, 153 (2008), Abstract;
Serum neutrophil gelatinase-associated lipocalin as a marker of renal function in non-diabetic patients with stage 2-4 chronic kidney disease: J. Malyszko, et al.; Ren. Fail. 30, 625 (2008), Abstract;
Urinary neutrophil gelatinase associated lipocalin (NGAL), a marker of tubular damage, is increased in patients with chronic heart failure: K. Damman, et al.; Eur. J. Heart Fail. 10, 997 (2008), Abstract;
Urinary neutrophil gelatinase-associated lipocalin (NGAL) is associated with severity of renal disease in proteinuric patients: D. Bolignano, et al.; Nephrol. Dial. Transplant. 23, 414 (2008), Abstract;
Urinary neutrophil gelatinase-associated lipocalin and acute kidney injury after cardiac surgery: G. Wagener, et al.; Am. J. Kidney Dis. 52, 425 (2008), Abstract;
Urinary NGAL in premature infants: A.P. Lavery, et al.; Pediatr. Res. 64, 423 (2008), Abstract;
Urine neutrophil gelatinase-associated lipocalin and interleukin-18 predict acute kidney injury after cardiac surgery: C. Xin, et al.; Ren. Fail. 30, 904 (2008), Abstract;
Urine NGAL predicts severity of acute kidney injury after cardiac surgery: a prospective study: M. Bennett, et al.; Clin. J. Am. Soc. Nephrol. 3, 665 (2008), Abstract;
Could neutrophil-gelatinase-associated lipocalin and cystatin C predict the development of contrast-induced nephropathy after percutaneous coronary interventions in patients with stable angina and normal serum creatinine values?: H. Bachorzewska-Gajewska, et al.; Kidney Blood Press. Res. 30, 408 (2007), Abstract;
Detection of subclinical tubular injury after renal transplantation: comparison of urine protein analysis with allograft histopathology: S. Schaub, et al.; Transplantation 84, 104 (2007), Abstract;
Neutrophil gelatinase-associated lipocalin (NGAL) correlations with cystatin C, serum creatinine and eGFR in patients with normal serum creatinine undergoing coronary angiography: H. Bachorzewska-Gajewska, et al.; Nephrol. Dial. Transplant. 22, 295 (2007), Abstract;
Neutrophil gelatinase-associated lipocalin in patients with autosomal-dominant polycystic kidney disease: D. Bolignano, et al.; Am. J. Nephrol. 27, 373 (2007), Abstract;
Neutrophil-gelatinase-associated lipocalin and renal function after percutaneous coronary interventions: H. Bachorzewska-Gajewska, et al.; Am. J. Nephrol. 26, 287 (2006), Abstract;

General Literature References

Application of emerging biomarkers of acute kidney injury in development of kidney-sparing polypeptide-based antibiotics: D. Burt, et al.; Drug Chem. Toxicol. 37, 204 (2014), Abstract;

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