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APC-PCI complex (human) monoclonal antibody (M36)

 
BPD-JST-001-38-02 200 µl 632.00 USD
 
BPD-JST-001-38-1 1 ml 2,834.00 USD
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Product Specification

Clone:M36
 
Host:Mouse
 
Isotype:IgG1κ
 
Immunogen:Mixture of PCI in complex with PSA (approx. 80%) and PCI cleaved from such a complex (approx. 20%).
 
UniProt ID:P05154 (human PCI)
 
Gene/Protein Identifier:5104 (Gene ID)
 
Species reactivity:Human
 
Specificity:BPD-JST-001-38 is specific for APC:PCI complex and cleaved PCI but has little reactivity towards native PCI. The dissociation constants (as determined by standard surface plasmon resonance technique) are: Kd= 4x10e-10 M for PCI in complex with APC and 2x10e-10 M for cleaved PCI. The value of Kd for native PCI is too low to measure, i.e. > 10e-5 M.
 
Applications:ELISA, WB
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Liquid. In 0.01 M phosphate buffer, pH 7.4, containing 0.14 M sodium chloride and 15 mM sodium azide.
 
Shipping:Blue Ice Not Frozen
 
Short Term Storage:+4°C
 
Long Term Storage:+4°C
 
Scientific Background:Activated Protein C (APC), a serine proteinase is inhibited by Protein C inhibitor (PCI). PCI belongs to a group of inhibitors sometimes referred to as serpines (serine proteinase inhibitors), and form 1:1 complexes with APC. PCI plasma concentration is approximately 4 µg/ml. The complex formation between APC and PCI proceeds at a slow rate, which is manifested by a long half-life for APC in plasma, wherein t1/2 is about 20 min. However, the rate of complex formation between APC and PCI is increased by heparin. Upon complex formation with APC, the serpin is cleaved in its so-called bait region, whereby a stable intermediate acyl complex is formed. With time, the intermediate acyl complex dissociates, whereby APC is regenerated and a proteolytically modified, i. e. cleaved, inactive serine proteinase inhibitor is formed.
 
BPD-JST-001-38 Fig1
Figure 1. Illustrates the calibration curve of a sandwich assay with BPD-JST-001-38 as the capture antibody and SA001RA as the biotinylated detection antibody.
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BPD-JST-001-38 Fig1

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