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Gliadin peptide monoclonal antibody (14D5)

BPD-HYB-314-01-02 200 µg 436.00 USD
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Product Specification

Alternative Name:alpha Gliadin
Immunogen:Synthetic peptide corresponding to aa Lys57-Glu65-[α-gliadin (58-73)] (KLQPFPQPELPYPQPQ) of Gliadin-related peptide.
Species reactivity:Plant
Applications:ELISA, WB
Application Notes:Detects a band of ~6 to 10kDa by Western blot.
Purity Detail:Protein A/G-affinity purified.
Formulation:Liquid. In 0.01M phosphate buffer, pH 7.4, containing 0.5M sodium chloride and 15mM sodium azide.
Shipping:Blue Ice Not Frozen
Short Term Storage:+4°C
Scientific Background:Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.
Gliadin peptide monoclonal antibody (14D5) Western blot
Figure 1. Gliadin peptide (detected with BPD-HYB 314-01) in rye (lane 1), barley (lane 2) and wheat (lane 3).
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Gliadin peptide monoclonal antibody (14D5) Western blot

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