Replaces Prod. #: BPD-HYB-156-02
Product Specification
| Alternative Name: | flu A virus |
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| Clone: | 5D8 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Unpurified influenza A virus (H1N1 A/PR/8/34) for primary intranasal immunization, boosted intravenously with purified influenza A virus disrupted with Triton X-100 for 45 min at 37°C. |
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| Species reactivity: | Influenza A virus
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| Applications: | ELISA, IF, WB
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| Purity Detail: | Protein A/G-affinity purified. |
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| Formulation: | Liquid. In 0.01M phosphate buffer, pH 7.4, containing 0.5M sodium chloride and 15mM sodium azide. |
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| Shipping: | Blue Ice Not Frozen |
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| Short Term Storage: | +4°C |
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| Scientific Background: | Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza A virus is a basic, phosphorylated multimeric protein consisting of 498 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle. |
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Figure 1: Immunofluorescent analysis of Vero cells using Influenza A Virus, mAb (5D8) diluted 1/1500 + secondary polyclonal goat anti-mouse Ig Fluor 555 conjugated antibody (Alexa A21424) 1/200 + Vectashield mounting medium with DAPI. DAKO IMAGEN Influenza Virus slides (S6112) were used and the entire infected Vero cells are seen.
Figure 2: Immunofluorescent analysis of Vero cells using Influenza A Virus, mAb (5D8) diluted 1/1000 + secondary polyclonal rabbit anti-mouse Ig FITC conjugated antibody (DAKO F0232) 1/20 + 1% Evans blue colors Influenza A nucleoprotein. DAKO IMAGEN Influenza Virus slides (S6112) were used and the entire infected Vero cells are seen.
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