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Influenza A virus monoclonal antibody (5D8)

 
BPD-HYB-156-01-02 200 µg 632.00 USD
 
BPD-HYB-156-01-1 1 mg 2,778.00 USD
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Replaces Prod. #: BPD-HYB-156-02

Product Specification

Alternative Name:flu A virus
 
Clone:5D8
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Unpurified influenza A virus (H1N1 A/PR/8/34) for primary intranasal immunization, boosted intravenously with purified influenza A virus disrupted with Triton X-100 for 45 min at 37°C.
 
Species reactivity:Influenza A virus
 
Applications:ELISA, IF, WB
 
Purity Detail:Protein A/G-affinity purified.
 
Formulation:Liquid. In 0.01M phosphate buffer, pH 7.4, containing 0.5M sodium chloride and 15mM sodium azide.
 
Shipping:Blue Ice Not Frozen
 
Short Term Storage:+4°C
 
Scientific Background:Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza A virus is a basic, phosphorylated multimeric protein consisting of 498 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle.
 
HYB 156-01  1  web
Figure 1: Immunofluorescent analysis of Vero cells using Influenza A Virus, mAb (5D8) diluted 1/1500 + secondary polyclonal goat anti-mouse Ig Fluor 555 conjugated antibody (Alexa A21424) 1/200 + Vectashield mounting medium with DAPI. DAKO IMAGEN Influenza Virus slides (S6112) were used and the entire infected Vero cells are seen.
HYB 156-01  2  web
Figure 2: Immunofluorescent analysis of Vero cells using Influenza A Virus, mAb (5D8) diluted 1/1000 + secondary polyclonal rabbit anti-mouse Ig FITC conjugated antibody (DAKO F0232) 1/20 + 1% Evans blue colors Influenza A nucleoprotein. DAKO IMAGEN Influenza Virus slides (S6112) were used and the entire infected Vero cells are seen.
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