Product Details
| Alternative Name: | Immunoglobulin E |
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| Source: | Purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. |
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| Formulation: | Liquid. In 0.01M phosphate buffer, pH 7.4, containing 0.15M sodium chloride. |
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| Purity: | ≥90% (SDS-PAGE) |
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| Purity Detail: | Protein L purified. The original antibody-producing B cells were obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B. Although BPD-DIA-HE1 is purified from a monoclonal hybridoma line derived from the antibody-producing donor cell, it must be handled as potentially infectious as all human material. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from fetal bovine serum. |
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| Applications: | ICC, FUNC
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| Application Notes: | Can be used as standard in IgE quantifying assays.
Functional Application: cell stimulation. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | -20°C |
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| Scientific Background: | IgE comes from a monoclonal cell line. This antibody is well-suited as a standard in IgE-quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: SDS-PAGE Analysis: MW marker (1), purified IgE (non-immune) (human) Protein (2) (185 kDa), and IgE, purified from serum from a myeloma patient (3).
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Product Literature References
IgE induces proliferation in human airway smooth muscle cells: role of MAPK and STAT3 pathways: N.S. Redhu, et al.; Allergy Asthma Clin. Immunol.
9, 41 (2013),
Application(s): Stimulation of human airway smooth muscle (HASM) cell’s with IgE,
Full Text
Double expression of CD34 and CD117 on bone marrow progenitors is a hallmark of the development of functional mast cell of Callithrix jacchus (common marmoset): S. Nunomura, et al.; Int. Immunol.
24, 593 (2012),
Application(s): Stimulation of mast cells (MC) with IgE,
Abstract;
Stimulation of human airway smooth muscle (HASM) cell’s with IgE: J. Balhara; (2012), (thesis),
Application(s): Stimulation of human airway smooth muscle (HASM) cell’s with IgE ,
Full Text
Aspergillus oryzae lectin induces anaphylactoid oedema and mast cell activation through its interaction with fucose of mast cell-bound non-specific IgE: K. Yamaki, et al.; Scand. J. Immunol.
74, 445 (2011),
Application(s): Investigation of Aspergillus oryzae involvement in allergic bronchopulmonary aspergillosis (ABPA),
Abstract;
Proinflammatory and Th2 cytokines regulate the high affinity IgE receptor (FcepsilonRI) and IgE-dependant activation of human airway smooth muscle cells: N.S. Redhu, et al.; PLoS One
4, e6153 (2009),
Application(s): Stimulation of human bronchial/tracheal smooth muscle (B/TSM) cells with IgE,
Abstract;
The study of surface properties of an IgE-sensitive aptasensor using an acoustic method: M. Šnejdárková, et al.; Anal. Bioanal. Chem.
390, 1087 (2008),
Abstract;
A pathogenic role for IgE in autoimmunity: bullous pemphigoid IgE reproduces the early phase of lesion development in human skin grafted to nu/nu mice: J.A. Fairley, et al.; J. Invest. Dermatol.
127, 2605 (2007),
Application(s): Control for IgE in skin graft on mice,
Abstract;
IgE modulates neutrophil survival in asthma: role of mitochondrial pathway: A.S. Saffar, et al.; J. Immunol.
178, 2535 (2007),
Application(s): Stimulation of neutrophils,
Abstract;
Memory T cell proliferation in cow's milk allergy after CD25+ regulatory T cell removal suggests a role for casein-specific cellular immunity in IgE-mediated but not in non-IgE-mediated cow's milk allergy: G.B.G. Sletten, et al.; Int. Arch. Allergy Immunol.
142, 190 (2007),
Abstract;
Validation of peptide epitope microarray experiments and extraction of quality data: T. Nahtman, et al.; J. Immunol. Methods
328, 1 (2007),
Application(s): Positive control in microarray technology,
Abstract;
Viability of allergy (IgE) detection using an alternative aptamer receptor and electrochemical means: K.I. Papamichael, et al.; Sensor Actuat. B-Chem.
121, 178 (2007),
Full Text
Expression of Fc(epsilon)-receptors by human acute myelogenous leukemia (AML) blasts: studies of high- and low- (CD23) affinity receptor expression and the effects of IgE-mediated receptor ligation on functional AML blast characteristics: Ø. Bruserud, et al.; Leuk. Res.
26, 515 (2002),
Application(s): Stimulation of acute myelogenous leukemia (AML) blasts,
Abstract;
