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IgE (non-immune) (human)

 
BPD-DIA-HE1-01 100 µg 424.00 USD
 
BPD-DIA-HE1-1 1 mg 2,011.00 USD
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Product Specification

Alternative Name:Immunoglobulin E
 
Source:Purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.
 
Formulation:Liquid. In 0.01M phosphate buffer, pH 7.4, containing 0.15M sodium chloride.
 
Purity:≥98% (SDS-PAGE)
 
Purity Detail:Protein L purified. The original antibody-producing B cells were obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B. Although BPD-DIA-HE1 is purified from a monoclonal hybridoma line derived from the antibody-producing donor cell, it must be handled as potentially infectious as all human material. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from fetal bovine serum.
 
Applications:ICC, FUNC
 
Application Notes:Can be used as standard in IgE quantifying assays.
Functional Application: cell stimulation.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:-20°C
 
Scientific Background:IgE comes from a monoclonal cell line. This antibody is well-suited as a standard in IgE-quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.
 
DIA HE1 web
Figure: SDS-PAGE Analysis: MW marker (1), purified IgE (non-immune) (human) Protein (2) (185 kDa), and IgE, purified from serum from a myeloma patient (3).
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DIA HE1 web

Product Literature References

IgE induces proliferation in human airway smooth muscle cells: role of MAPK and STAT3 pathways: N.S. Redhu, et al.; Allergy Asthma Clin. Immunol. 9, 41 (2013), Application(s): Stimulation of human airway smooth muscle (HASM) cell’s with IgE, Full Text
Double expression of CD34 and CD117 on bone marrow progenitors is a hallmark of the development of functional mast cell of Callithrix jacchus (common marmoset): S. Nunomura, et al.; Int. Immunol. 24, 593 (2012), Application(s): Stimulation of mast cells (MC) with IgE, Abstract;
Stimulation of human airway smooth muscle (HASM) cell’s with IgE: J. Balhara; (2012), (thesis), Application(s): Stimulation of human airway smooth muscle (HASM) cell’s with IgE , Full Text
Aspergillus oryzae lectin induces anaphylactoid oedema and mast cell activation through its interaction with fucose of mast cell-bound non-specific IgE: K. Yamaki, et al.; Scand. J. Immunol. 74, 445 (2011), Application(s): Investigation of Aspergillus oryzae involvement in allergic bronchopulmonary aspergillosis (ABPA), Abstract;
Proinflammatory and Th2 cytokines regulate the high affinity IgE receptor (FcepsilonRI) and IgE-dependant activation of human airway smooth muscle cells: N.S. Redhu, et al.; PLoS One 4, e6153 (2009), Application(s): Stimulation of human bronchial/tracheal smooth muscle (B/TSM) cells with IgE, Abstract;
The study of surface properties of an IgE-sensitive aptasensor using an acoustic method: M. Šnejdárková, et al.; Anal. Bioanal. Chem. 390, 1087 (2008), Abstract;
A pathogenic role for IgE in autoimmunity: bullous pemphigoid IgE reproduces the early phase of lesion development in human skin grafted to nu/nu mice: J.A. Fairley, et al.; J. Invest. Dermatol. 127, 2605 (2007), Application(s): Control for IgE in skin graft on mice, Abstract;
IgE modulates neutrophil survival in asthma: role of mitochondrial pathway: A.S. Saffar, et al.; J. Immunol. 178, 2535 (2007), Application(s): Stimulation of neutrophils, Abstract;
Memory T cell proliferation in cow's milk allergy after CD25+ regulatory T cell removal suggests a role for casein-specific cellular immunity in IgE-mediated but not in non-IgE-mediated cow's milk allergy: G.B.G. Sletten, et al.; Int. Arch. Allergy Immunol. 142, 190 (2007), Abstract;
Validation of peptide epitope microarray experiments and extraction of quality data: T. Nahtman, et al.; J. Immunol. Methods 328, 1 (2007), Application(s): Positive control in microarray technology, Abstract;
Viability of allergy (IgE) detection using an alternative aptamer receptor and electrochemical means: K.I. Papamichael, et al.; Sensor Actuat. B-Chem. 121, 178 (2007), Full Text
Expression of Fc(epsilon)-receptors by human acute myelogenous leukemia (AML) blasts: studies of high- and low- (CD23) affinity receptor expression and the effects of IgE-mediated receptor ligation on functional AML blast characteristics: Ø. Bruserud, et al.; Leuk. Res. 26, 515 (2002), Application(s): Stimulation of acute myelogenous leukemia (AML) blasts, Abstract;

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