Orai1 (extracellular) (human), pAb



BML-SA674-0050	50 µl	291.00 USD

BML-SA674-0200	200 µl	455.00 USD

Product Specification

Alternative Name:	Calcium release-activated calcium channel protein 1, Transmembrane protein 142A

Concentration:	0.8mg/ml after reconstitution.

Purity Detail:	Affinity purified.

Formulation:	Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide.

Immunogen:	Synthetic peptide corresponding to aa 203-214 (K²⁰³KQPGQPRPTSK²¹⁴) of human Orai1.

Source/Host:	From rabbit.

Reconstitution:	Reconstitute with 50μl or 200μl distilled water. Further dilutions should be made with medium containing a carrier protein such as 1% BSA.

Specificity:	Recognizes human Orai1. Does not cross-react with mouse or rat Orai1.

Application:	Flow Cytometry Western Blot (1:200)

Long Term Storage:	-20°C

Use/Stability:	The antibody solution can be stored at +4°C for up to 2 weeks.

Handling:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Blocking Peptide:	40μg included. Lyophilized. Reconstitute with 100μl distilled water.

Miscellaneous/General:	Cytosolic calcium (Ca²⁺) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation. The mechanism controlling intracellular Ca²⁺ level influx either by the calcium-release-activated Ca²⁺ channels (CRAC), or from intracellular stores, has become of great interest to researchers. Several key players of the store operated complex have been identified. Orai1 (also known as CRACM1) acts as the store operated calcium channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca²⁺ sensor. The formation of functional channels by Orai1 requires the presence of both Orai1 and STIM1 proteins working as a complex that involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum.

Background / Technical Information:	Preadsorption control: 1µg peptide per 1µg antibody. Prepare 2 tubes. In the first, dilute the necessary amount of antibody in 200-500µl of PBS, 1% BSA and 0.025% sodium azide. Prepare the second tube identically to the first with the addition of the appropriate amount of peptide. Incubate at room temperature for 1 hr. Centrifuge both tubes at 10,000Xg for 5 min. Use the supernatants from tubes 1 and 2 for parallel experiments.

Figure: Western blot analysis of Jurkat cell lysate. Lane 1: PAb to Orai1 (Extracellular) (human) (Prod. No. BML-SA674) Lane 2: PAb to Orai1 (Extracellular) (human) preincubated with the control peptide.

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General Literature References

A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function: S. Feske, et al.; Nature 441, 179 (2006), Abstract;

Ca2+ store depletion causes STIM1 to accumulate in ER regions closely associated with the plasma membrane: M.M. Wu, et al.; J. Cell Biol. 174, 803 (2006), Abstract; Full Text

Calcium signaling in non-excitable cells: Ca2+ release and influx are independent events linked to two plasma membrane Ca2+ entry channels: R. Chakrabarti & R. Chakrabarti; J. Cell Biochem. 99, 1503 (2006), Abstract;

Calcium: You lose some, you gain some: J. Pickett; Nature Rev. Mol. Cell Biol. 7, 794 (2006),

The elementary unit of store-operated Ca2+ entry: local activation of CRAC channels by STIM1 at ER-plasma membrane junctions: R.M. Luik, et al.; J. Cell. Biol. 174, 815 (2006), Abstract; Full Text

Stability and instability of regulation of intracellular calcium: D.A. Eisner, et al.; Exp. Physiol. 90, 3 (2005), Abstract; Full Text