Fluor-de-Lys^®-Green HDAC fluorometric activity assay kit



BML-AK530-0001	1 Kit	335.00 USD

An improved Fluor de Lys^® HDAC assay with Fluor de Lys^®-Green, a new substrate offering higher sensitivity and an excitation and emission (485/530nm) that avoids quenching and fluorescent interference from compounds absorbing in the near UV and blue range.The Fluor de Lys^®-Green HDAC assay is a complete kit for measuring histone deacetylase (HDAC) activity in cell or nuclear extracts, immunoprecipitates or purified enzymes. It comes in a convenient 96-well format, with all reagents necessary for fluorescent HDAC or sirtuin activity measurements and calibration of the assay.

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Figure 1: Reaction scheme of the Fluor de Lys^®-Green HDAC assay kit. Deacetylation of the substrate sensitizes it to the developer, which then generates a fluorophore (lily-symbol).

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Product Specification

Alternative Name:	Histone deacetylase green fluorescent assay kit

Quantity:	96 assays

Kit/Set Contains:	Nuclear Extract from HeLa Cells (human cervical cancer cell line) (Prod. No. BML-KI140) (100μl; in 0.1M potassium chloride, 20mM HEPES/NaOH, 20% (v/v) glycerol, 0.2mM EDTA, 0.5mM DTT, 0.5mM PMSF) Storage: -70°C. Fluor de Lys^®-Green Substrate (Prod. No. BML-KI572) (50μl; 50mM in DMSO) Storage: -70°C. Fluor de Lys^® Developer Concentrate (20X) (Prod. No. BML-KI105) (300μl; 20x stock solution; dilute in assay buffer before use) Storage: -70°C. Trichostatin A (Prod. No. BML-GR309) (100μl; 0.2mM in DMSO) Storage: -70°C. Fluor de Lys^®-Green Deacetylated Standard (Prod. No. BML-KI573) (30μl; 1mM in DMSO) Storage: -70°C. NAD⁺ (Sirtuin Substrate) (Prod. No. BML-KI282) (500μl; 50mM in 50mM TRIS, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride) Storage: -70°C. Nicotinamide (Prod. No. BML-KI283) (500μl; 50mM in 50mM TRIS, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride) Storage: -70°C. HDAC Assay Buffer (Prod. No. BML-KI143) (20ml; 50mM TRIS, pH 8.0, 137mM sodium chloride, 2.7mM potassium chloride, 1mM magnesium chloride) Storage: -70°C. 96 Well Plates (Prod. No. BML-KI101) Storage: Room temperature White NBS Microplate (Prod. No. BML-KI571) Storage: Room temperature

Long Term Storage:	-80°C

Handling:	Store all components (except microtiter plates) at -70°C. Unused HeLa Nuclear Extract should be refrozen quickly, if possible snap freeze in liquid nitrogen.

Miscellaneous/General:	The original Fluor de Lys^® HDAC fluorometric activity assay kit revolutionized HDAC and sirtuin screening by providing a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of lysine deacetylases.

Background / Technical Information:	For the Manual please click here.