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ISG15 activating enzyme E1 (human), (recombinant) (His-tag)

 
BML-UW9955-0025 25 µg 241.00 USD
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Product Specification

MW:113 kDa
 
Source:Produced in E. coli. Human ISG15 E1 is fused at the C-terminus to a His-tag.
 
UniProt ID:P41226
 
Formulation:Liquid. In 50mM TRIS-Cl, pH 7.5, 50mM NaCl, 1mM DTT.
 
Purity:≥95% (SDS-PAGE)
 
Purity Detail:Purified by Ni-affinity chromatography.
 
Application Notes:Uses:
Activation of ISG15 for ISGylation of specific proteins in presence of dedicated E2 conjugating/E3 ligase enzymes.
ISGylation pathway studies. Test proteins for ISG15 E3 ligase activity.
Test proteins as potential targets for ISG15 modification.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:The interferon (IFN)-α/β-induced ISG15 E1 activating enzyme, also known as known as Ube1L, is a single-subunit, ATP dependent enzyme that facilitates conjugation of ISG15 to cellular proteins via the ISGylation pathway.
 

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