hHR6A (human), (recombinant) (His-tag)

Ubiquitin-conjugating DNA repair enzyme.

Alternative Name:	UbcH2A

Source/Host:	Produced in E. coli.

Long Term Storage:	-80°C

Miscellaneous/General:	The family of UBC enzymes plays a central role in the ubiquitin pathway. Genetic studies with the S.cerevisiae Rad6 protein demonstrate a pleiotropic phenotype, including defects in DNA repair, proteolysis of N-end rule protein substrates, cellular proliferation, cell cycle progression and an inability to sporulate, which are all dependent on its ubiquitin-conjugating activity. Rad6 can ubiquitinylate histones in vitro, and ubiquitinylation of histone H2B in S. cerevisiae is Rad6 dependent. In mice and humans, two homologues of RAD6 have been identified - hHR6A (homologue of RAD6A) and hHR6B (homologue of RAD6B). UbcH2A is phosphorylated in vitro by CDK-1 and -2 on serine residue 120, which is conserved in all hHR6A homologs, leading to an increase in the ubiquitin-conjugating activity of this enzyme.

Background / Technical Information:	Please click here for the comprehensive product datasheet.