Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

UbcH13/Mms2 (human), (recombinant) (His-tag)

 
BML-UW9565-0100 100 µg 258.00 USD
Do you need bulk/larger quantities?
 
A heterodimer composed of the catalytically active ubiquitin-conjugating enzyme UbcH13 and its catalytically inactive paralog hMms2, which lacks an active site cysteine residue.

Product Specification

MW:~18.4kDa
 
Source:Produced in E. coli.
 
UniProt ID:P61088
 
Formulation:Liquid. In 50mM HEPES, pH 8.0, 50mM sodium chloride, 10% glycerol, 1mM DTT.
 
Purity:≥95% (SDS-PAGE)
 
Application Notes:Typical enzyme concentration to support conjugation in vitro is 100nM to 1µM depending upon conditions.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles.
 
Scientific Background:The UbcH13/Mms2 complex catalyzes synthesis of Lys-linked, ubiquitin chains, which serve as non-proteolytic tags involved in error-free post-replicative DNA repair and NF-κB signal transduction.
 
BML-UW9565 SDS-PAGE
Due to similarities in molecular weight (18.4kDa and 16.4kDa) it is difficult to separate the two components on SDS-PAGE under standard conditions. UW9565 has been characterised in a thiolester assay [in the presence (A) and absence (B) of ATP] using biotinylated ubiquitin (product code UW8705) as shown opposite. Major bands observed are for biotinylated ubiquitin and the biotinyl-ubiquitin thiol ester linked UbcH13 as marked. Please note that, due to the efficiency of ubiquitin transfer under the conditions used, it is not possible to observe the presence of the biotinyl-ubiquitin thiol ester linked E1.
Please mouse over
BML-UW9565 SDS-PAGE

General Literature References

An NMR-based model of the ubiquitinbound human ubiquitin conjugation complex Mms2: S. McKenna et al.; J. Biol. Chem. 278, 13151 (2003),
In vitro assembly and recognition of Lys-63 polyubiquitin chains.: Hofmann, R.M. and Pickart, C.M. ; J. Biol. Chem. 276, 27936-27943 (2001),
Noncanonical MMS2-encoded ubiquitin conjugating enzyme functions in assembly of novel polyubiquitin chains for DNA repair.: Hofmann, R.M. and Pickart, C.M.; Cell 96, 645-653 (1999),

Related Literature

Brochures
Stem Cells
Stem Cells
Download as PDF

Catalogs
Ubiquitin & UBL Signaling Catalog
Ubiquitin & UBL Signaling Catalog
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,