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pro-NEDD8 (human), (recombinant) (His-tag)

 
BML-UW9220-0500 500 µg 186.00 USD
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BML-UW9220 is a recombinant protein produced in E. coli corresponding to the human pro-NEDD8 sequence (Accession number: Q15843) encompassing an N-terminal His6-tag within a leader sequence. This product contains a C-terminal pentapeptide extension (GGLRQ), which may subsequently be cleaved to expose the C-terminal di-glycine of the mature form.

Product Specification

MW:~13.9 kDa
 
Source:Produced in E. coli.
 
UniProt ID:Q15843
 
Formulation:Liquid. In 20mM HEPES, pH 8.0, containing 300mM sodium chloride and 1mM DTT.
 
Purity:≥95% (SDS-PAGE)
 
Application Notes:For regulation and processing studies.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
 
Scientific Background:NEDD8 is a ubiquitin-like molecule, sharing ~60% identity with ubiquitin at the amino acid level, that can be covalently conjugated (neddylation) to a limited number of cellular proteins in a fashion similar to that of ubiquitin. The C-terminus of pro-NEDD8, GGLRQ, is efficiently processed to expose glycine76 in the mature form. This processing is required for conjugation to target proteins via a range of specific activating and conjugating enzymes. The E1 activating-enzyme complex is composed of two subunits, APP-BP1 and UBA3, whilst the E2 conjugating-enzyme is the ubiquitin E2 UbcH12.
The expression of NEDD8 is essential for growth in most organisms where it has been shown to modify the cullin subunit of cullin-based ubiquitin-protein ligases (E3s). Neddylation acts to regulate the function of these E3s and organisms with lesions in the neddylation process exhibit severe growth defects.

Previously, it was believed that the cullins were the only substrates for NEDDylation, however, it is now recognised that there are a number of such proteins, including the von Hippel-Lindau (VHL) tumour suppressor gene and the tumour suppressor and transcriptional regulator p53 amongst others.
 
UW9220 SDS-PAGE
Coomassie stained gel. Lane 1: MW markers (top to bottom) 66, 45, 36, 29, 24, 20 and 16. Lane 2: BML-UW9220 (10ug).
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UW9220 SDS-PAGE

General Literature References

Neddylating the guardian; Mdm2 catalyzed conjugation of Nedd8 to p53: J.W. Harper; Cell 118, 2 (2004), Abstract;
pVHL modification by NEDD8 is required for fibronectin matrix assembly and suppression of tumor development: N.H. Stickle, et al.; Mol. Cell Biol. 24, 3251 (2004), Abstract;
Regulation of cullin-based ubiquitin ligases by the Nedd8/RUB ubiquitin-like proteins: G. Parry & M. Estelle; Semin. Cell Dev. Biol. 15, 221 (2004), Abstract;
Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway: L. Gong & E.T. Yeh; J. Biol. Chem. 274, 12036 (1999), Abstract;

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