Product Details
| MW: | ~18 kDa |
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| Source: | Produced in E. coli. |
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| UniProt ID: | O14933 |
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| Formulation: | Liquid. In 20mM TRIS-HCl, pH 8.0, containing 0.5mM DTT. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Biological Activity: | This enzyme supports ubiquitinylation in vitro. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | UbcH8 is a class I ubiquitin-conjugating (E2) enzyme highly similar in structure to UbcH7. UbcH8 has been demonstrated to act in conjunction with Parkin, a ring-finger-containing E3 ubiquitin protein ligase implicated in the pathogenesis of autosomal recessive familial Parkinson's Disease, and with Staring, an ring-finger E3 ligase responsible for ubiquitinylation of syntaxin 1. UbcH8 is a primary target gene of interferon-α and γ, and recent studies have indicated that UbcH8 is the E2 enzyme for the ubiquitin-like protein ISG15 (also known as UCRP). These studies suggest overlap between the ISG15 and ubiquitin conjugation pathways, and have important implications for understanding the regulation and function of ISG15 in the IFN α/β response. |
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| Technical Info/Product Notes: | Typical enzyme concentration to support conjugation in vitro is 100nM to 1µM depending upon conditions. |
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| Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE analysis of Prod. No. BML-UW9135: Lane 1: MW marker, Lane 2: 2µg UbcH8 (human), (recombinant) (His-tag), Lane 3: MW marker.
Western Blot analysis of BML-UW9135: Lane 1: MW Marker, Lane 2: 100ng of UbcH8 (human), (recombinant) (His-tag) probed with Anti-6X His-tag® antibody.
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Product Literature References
The E3 ubiquitin ligase ARIH1 promotes antiviral immunity and autoimmunity by inducing mono-ISGylation and oligomerization of cGAS: T.C. Xiong, et al.; Nat. Commun.
13, 5973 (2022),
Abstract;
