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Ubiquitin, (biotinylated)

 
BML-UW8705-0100 100 µg 160.00 USD
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Biotinylated ubiquitin (prepared by the action of biotinamidocaproic acid N-hydroxysuccinimide ester on ubiquitin) is useful for studying ubiquitinylation in vitro, where it represents an alternative to radio-iodinated ubiquitin. This product contains a number of multiply biotinylated ubiquitin species, which has proven itself to be a reagent of considerable utility particularly for carrying out E1/E2-mediated thiolester assays.

Product Specification

Source:Produced in E. coli.
 
UniProt ID:P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52)
 
Formulation:Lyophilized from Acetonitrile:water (1:1).
 
Purity Detail:Mass spectral analysis shows presence of [biotinyl]1-5-ubiquitin with [biotinyl]2-4-ubiquitin being the predominate species.
 
Appearance:White solid.
 
Application Notes:Biotinylated ubiquitin is activated by E1 and is a competent substrate for E2 and E3 enzymes. The biotinyl moiety also provides a potential route to affinity purification of labeled species.
 
Solubility:In order to ensure full solubility it is recommended that the material be dissolved in 5µL DMSO prior to dilution with the buffer of choice to the desired working concentration.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles.
 

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For Research Use Only. Not for use in diagnostic procedures.
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