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Ubiquitin (bovine), (native) (methylated)

 
BML-UW8555-0001 1 mg 128.00 USD
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Octa-dimethyl-ubiquitin: (CH3)2-MQIFVK*TLTGK*TITLEVEPSDTIENV K*AK*IQDK*EGIPPDQQRLIFAGK*QLEDGR TLSDYNIQK*ESTLHLVLRLRGG-OH where K* = (ε-dimethyl)lysyl.

Product Specification

MW:~8.7kDa
 
Source:Native bovine.
 
UniProt ID:P0CG53
 
Formulation:Lyophilized in HPLC-grade water.
 
Purity:≥95% (SDS-PAGE)
 
Appearance:White solid.
 
Application Notes:Simplifies kinetics for E1-catalyzed active thioester formation.

Typical concentrations for non-rate limiting support of in vitro conjugation reactions lie in the range 200μM to 1mM depending upon individual conditions.
 
Solubility:Soluble and stable in DMSO and aqueous buffers up to 100 mg/mL. The solid will dissolve with gentle vortexing at the desired concentration.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Store solid cold and dry at -20C; stable for at least one year. Store solutions at -20C for up to three months. The protein is stable to multiple freeze-thaw cycles.
 
Scientific Background:Reductive methylation of the seven internal lysine ε-amino groups as well as the α-amino group to give, in this case, the octa-dimethyl derivative renders ubiquitin unable to form multi-ubiquitin chains via lysine linkages. However, the ability of this methylated ubiquitin to form an active C-terminal thioester is preserved and it may be transferred by ubiquitin-activating and conjugating enzymes (E1 and E2s) to lysine residues within substrate proteins to yield monoubiquitinylated products.
 
BML-UW8555 SDS-PAGE
SDS-PAGE Analysis: Lane 1: MW Marker, Lane 2: 0.5 μg, Lane 3: 1.0 μg, Lane 4: 2.0 μg, Lane 5: 5.0 μg of Purified Ubiquitin (methylated) (bovine), (native) (Prod. No. BML-UW8555).
BML-UW8555 Mass Spec
Mass spectrum analysis by electrospray ionization mass spectrometry, (see image) indicates the octa-dimethylated ubiquitin molecular weight at 8790 Da (± 1.6 Da), doubly charged species present at 6671.7 Da. This is fully consistent with the anticipated molecular weight, given that for parent ubiquitin a molecular weight of 8565.4 Da (± 1.6 Da) is observed under identical conditions. There is no un-derivatized ubiquitin present. The signal observed at 8675.3 Da is attributable to a minor contaminant in the starting ubiquitin and is not due to the presence of octa-monomethylated ubiquitin. All other minor signals observed are at background level and are not indicative of protein artifacts.
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BML-UW8555 SDS-PAGE BML-UW8555 Mass Spec

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